Pararhodobacter marinus sp. nov., isolated from deep-sea water of the Indian Ocean

A taxonomic study was carried out on strain CIC4N-9(T), which was isolated from deep-sea water of the Indian Ocean. The bacterium was Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and non-motile. Growth was observed at salinities of 0-9% and at temperatures of 4-41 degrees C. The isolate was able to degrade gelatin but not aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CIC4N-9(T) belonged to the genus Pararhodobacter, with the highest sequence similarity to the only recognized species, Pararhodobacter aggregans D1-19(T) (96.9 %). The average nucleotide identity and estimated DNA-DNA hybridization values between strain CIC4N-9(T) and P. aggregans D1-19(T) were 80.4 and 23.0 %, respectively. The principal fatty acids were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c), C-16:0, C-18:1 omega 7c 11methyl, C-18:0 and C-17:0. The G+C content of the chromosomal DNA was 66.8 mol%. The sole respiratory quinone was determined to be Q-10. Phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, two unknown phospholipids, four unknown aminolipids and one unknown polar lipid were present. The combined genotypic and phenotypic data show that strain CIC4N-9(T) represents a novel species within the genus Pararhodobacter, for which the name Pararhodobacter marinus sp. nov. is proposed. The type strain is CIC4N-9(T)(=MCCC 1A01225(T)=KCTC 5233(T)).