Vancomycin Hydrochloride Loaded Hydroxyapatite Mesoporous Microspheres with Micro/Nano Surface Structure to Increase Osteogenic Differentiation and Antibacterial Ability

As infection induced by the implant will lead to operation failure, the implant material must be endowed with certain antibacterial properties. Hydroxyapatite (HA) mesoporous microspheres have been widely used in bone repair due to their advantages, including simple synthesis, good osteogenic properties and drug loading capacity. In this study, vancomycin IP: 49 249.253.194 On: Wed, 22 Sep 2021 06:32:24 hydrochloride-loaded mesoporous hydroxyapatite microspheres with micro/nanosurface structures were synthesized to Copyright: American Scient fic Publishers increase osteogenic differentiation and antibacterial ability. Phtic acid (IP6) was used as a template to prepare meso-Delivered by Ingenta porous hydroxyapatite microspheres composed of fibres, flakes and smooth surfaces by the hydrothermal homogeneous precipitation method, and the corresponding specific surface areas were 65.20 m(2)/g, 75.13 m(2)/g and 71.27 m(2)/g, respec-tively. Vancomycin hydrochloride (Van) was used as the drug model to study the drug loading and release characteristics of the microspheres, as well as the in vitro antibacterial properties after treatment. In addition, during cocultivation with MC3T3-E1 preosteoblasts, HA microspheres assembled via flakes exhibited better cell compatibility, which promoted cell proliferation, alkaline phosphatase (ALP) activity, and the formation of calcium nodules and increased the expression of osteogenic differentiation-related proteins such as Runx-2, osteopontin (OPN) and collagen I (COL I). These results indi-cated that the HA microspheres prepared in this experiment have broad application prospects in drug delivery systems and bone repair.