Fourier transform infrared (FT-IR) spectroscopy typing: a real-time analysis of an outbreak by carbapenem-resistantKlebsiella pneumoniae

被引:13
|
作者
Silva, Liliana [1 ,2 ]
Rodrigues, Carla [1 ,3 ]
Lira, Agostinho [4 ]
Leao, Mariana [4 ]
Mota, Margarida [4 ]
Lopes, Paulo [4 ]
Novais, Angela [1 ]
Peixe, Luisa [1 ]
机构
[1] Univ Porto, Fac Farm, Lab Microbiol, UCIBIO REQUIMTE, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto, Portugal
[2] Inst Politecn Castelo Branco, ESALD, Castelo Branco, Portugal
[3] Inst Pasteur, Biodivers & Epidmiol Bacterial Pathogens, Paris, France
[4] Ctr Hosp Vila Nova de Gaia Espinho, Serv Patol Clin, CHVNG E, Vila Nova De Gaia, Portugal
关键词
Bacterial typing; Nosocomial infection; Carbapenemases; KPC-3; Capsule type; Capsular polysaccharides;
D O I
10.1007/s10096-020-03956-y
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The spread of multidrug-resistant (MDR)Klebsiella pneumoniaein the nosocomial setting represents a big challenge to infection control teams. We have recently developed a simple spectroscopic-based method with excellent accuracy, turnaround time and cost-effectiveness (Rodrigues et al. mSystems 2020) for bacterial typing. Here, we applied our method in a real clinical context to support early identification of an outbreak involving KPC-3-producingK. pneumoniaeST147 isolates. Our results further support that attenuated total reflectance Fourier transform infrared (FT-IR) spectroscopy can provide enough information to support early and adequate infection control measures and therapeutic choices in the context of nosocomial outbreaks and hospital surveillance.
引用
收藏
页码:2471 / 2475
页数:5
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