Quantitative analysis of biosurfactants using Fourier Transform Infrared (FT-IR) spectroscopy

被引：15
|
作者：
Gartshore, J [1 ]
Lim, YC [1 ]
Cooper, DG [1 ]
机构：
[1] McGill Univ, Dept Chem Engn, Montreal, PQ H3A 2B2, Canada
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 02期
基金：
加拿大自然科学与工程研究理事会;
关键词：
biosurfactants; infrared spectroscopy; quantification;
D O I：
10.1023/A:1005670031432
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A quick and simple technique has been developed that is suitable to quantify the concentration of most types of biosurfactants in a typical growth medium using infrared spectroscopy. The area of the peak due to the carbonyl bond of the biosurfactant was measured and compared to the area of the nitrile peak in an internal standard (anthracenecarbonitrile). This ratio showed a linear trend with the concentration of biosurfactant in the medium for concentrations from 0.2 to 3.2 g biosurfactant l(-1). The presence of hydrocarbons in the media and variations in the pH of the media did not interfere with the measurements.
引用
收藏
页码：169 / 172
页数：4
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