Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity

被引：25
|
作者：
Valentini, G
D'Andrea, C
Comelli, D
Pifferi, A
Taroni, P
Torricelli, A
Cubeddu, R
Battaglia, C
Consolandi, C
Salani, G
Rossi-Bernardi, L
De Bellis, G
机构：
[1] Politecn Milan, INFM, Dipartimento Fis, I-20133 Milan, Italy
[2] CNR, Ctr Elettr Quantist & Strumentaz Elettr, I-20133 Milan, Italy
[3] Univ Milan, Dipartimento Sci & Tecnol Biomed, I-20090 Milan, Italy
[4] CNR, Ist Tecnol Biomed Avanzute, I-20090 Milan, Italy
来源：
OPTICS LETTERS | 2000年 / 25卷 / 22期
关键词：
D O I：
10.1364/OL.25.001648
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection. (C) 2000 Optical Society of America OCIS codes: 170.6920, 170.0110, 260.2510, 170.3880.
引用
收藏
页码：1648 / 1650
页数：3
相关论文
共 50 条
  • [1] DNA microarray reading by an intensified CCD camera with picosecond time resolution
    Cubeddu, R
    D'Andrea, C
    Pifferi, A
    Taroni, P
    Torricelli, A
    Valentini, G
    Battaglia, C
    Consolandi, C
    Rossi-Bernardi, L
    Salani, G
    De Bellis, G
    BIOMEDICAL TOPICAL MEETINGS, TECHNICAL DIGEST, 2000, 38 : 120 - 122
  • [2] Ultimate sensitivity of time-resolved opto-acoustic detection
    Oraevsky, A
    Karabutov, A
    BIOMEDICAL OPTOACOUSTICS, 2000, 3916 : 228 - 239
  • [3] TIME-RESOLVED PHOTOMETRY USING A CCD
    HOWELL, SB
    JACOBY, GH
    PUBLICATIONS OF THE ASTRONOMICAL SOCIETY OF THE PACIFIC, 1986, 98 (606) : 802 - 808
  • [4] TIME-RESOLVED CCD PHOTOMETRY OF AN ENSEMBLE OF STARS
    GILLILAND, RL
    BROWN, TM
    PUBLICATIONS OF THE ASTRONOMICAL SOCIETY OF THE PACIFIC, 1988, 100 (628) : 754 - 765
  • [5] Direct modulation of the effective sensitivity of a CCD detector: a new approach to time-resolved fluorescence imaging
    Mitchell, AC
    Wall, JE
    Murray, JG
    Morgan, CG
    JOURNAL OF MICROSCOPY-OXFORD, 2002, 206 (03): : 225 - 232
  • [6] HIGH-PRECISION TIME-RESOLVED CCD PHOTOMETRY
    KJELDSEN, H
    FRANDSEN, S
    PUBLICATIONS OF THE ASTRONOMICAL SOCIETY OF THE PACIFIC, 1992, 104 (676) : 413 - 434
  • [7] Time-resolved diffusing wave spectroscopy with a CCD camera
    Zarychta, Katarzyna
    Tinet, Eric
    Azizi, Leila
    Avrillier, Sigrid
    Ettori, Dominique
    Tualle, Jean-Michel
    OPTICS EXPRESS, 2010, 18 (16): : 16289 - 16301
  • [8] A simplified system for reading time-resolved photoluminescence
    Yasuda, H
    Ishidoya, T
    JOURNAL OF RADIATION RESEARCH, 2002, 43 : S63 - S65
  • [9] Time-resolved Forster-resonance-energy-transfer DNA assay on an active CMOS microarray
    Schwartz, David Eric
    Gong, Ping
    Shepard, Kenneth L.
    BIOSENSORS & BIOELECTRONICS, 2008, 24 (03): : 383 - 390
  • [10] CCD Time-Resolved Photometry of Four Newly Discovered CVs
    Liu W Hu J Y Beijing Astronomical Observatory National Astronomical Observatories The Chinese Academy of Sciences Beijing China Email liuwuclass bao ac cn
    云南天文台台刊, 1999, (S1) : 387 - 391
12345