Towards an improved qualitative and quantitative determination of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by HPLC coupled to ICP-MS

被引：14

作者：

Jitaru, Petru ^{[1
,2
]}

Cozzi, Giulio ^{[1
]}

Seraglia, Roberta ^{[3
]}

Traldi, Pietro ^{[3
]}

Cescon, Paolo ^{[1
,4
]}

Barbante, Carlo ^{[1
,4
]}

机构：

[1] Inst Dynam Environm Proc IDPA CNR, I-30123 Venice, Italy

[2] Inst Polytech LaSalle Beauvais, Dept Sci & Tech Agroind, F-60026 Beauvais, France

[3] Inst Sci & Mol Technol ISTM CNR, I-35100 Padua, Italy

[4] Univ Venice Ca Foscari, Dept Environm Sci, I-30123 Venice, Italy

来源：

ANALYTICAL METHODS | 2010年 / 2卷 / 09期

关键词：

ANION-EXCHANGE CHROMATOGRAPHY; SPECIATION ANALYSIS; MASS-SPECTROMETRY; QUADRUPOLE MS; AFFINITY HPLC; SELENIUM; PLASMA; ALBUMIN; PROTEINS; COLUMNS;

D O I：

10.1039/c0ay00173b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper deals primarily with the validation of a clean-up procedure based on anion exchange solid-phase extraction for the accurate determination of glutathione peroxidase (GPx), selenoprotein P (SelP) and selenoalbumin (SeAlb) in human serum by affinity HPLC (AF-HPLC) coupled to inductively coupled plasma-mass spectrometry (ICP-MS). In addition, the identification and the purity assessment of the GPx, SelP and SeAlb peak fractions separated by AF-HPLC is addressed by their analysis using matrix assisted laser desorption ionization-time of flight mass spectrometry.

引用

页码：1382 / 1387

页数：6

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