Towards an improved qualitative and quantitative determination of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by HPLC coupled to ICP-MS

被引：14
|
作者：
Jitaru, Petru [1 ,2 ]
Cozzi, Giulio [1 ]
Seraglia, Roberta [3 ]
Traldi, Pietro [3 ]
Cescon, Paolo [1 ,4 ]
Barbante, Carlo [1 ,4 ]
机构：
[1] Inst Dynam Environm Proc IDPA CNR, I-30123 Venice, Italy
[2] Inst Polytech LaSalle Beauvais, Dept Sci & Tech Agroind, F-60026 Beauvais, France
[3] Inst Sci & Mol Technol ISTM CNR, I-35100 Padua, Italy
[4] Univ Venice Ca Foscari, Dept Environm Sci, I-30123 Venice, Italy
来源：
ANALYTICAL METHODS | 2010年 / 2卷 / 09期
关键词：
ANION-EXCHANGE CHROMATOGRAPHY; SPECIATION ANALYSIS; MASS-SPECTROMETRY; QUADRUPOLE MS; AFFINITY HPLC; SELENIUM; PLASMA; ALBUMIN; PROTEINS; COLUMNS;
D O I：
10.1039/c0ay00173b
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This paper deals primarily with the validation of a clean-up procedure based on anion exchange solid-phase extraction for the accurate determination of glutathione peroxidase (GPx), selenoprotein P (SelP) and selenoalbumin (SeAlb) in human serum by affinity HPLC (AF-HPLC) coupled to inductively coupled plasma-mass spectrometry (ICP-MS). In addition, the identification and the purity assessment of the GPx, SelP and SeAlb peak fractions separated by AF-HPLC is addressed by their analysis using matrix assisted laser desorption ionization-time of flight mass spectrometry.
引用
收藏
页码：1382 / 1387
页数：6
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