The protein phosphatase type 2A alpha throughout the dephosphorylation of serine/threonine residues of phosphoproteins is involved in numerous cellular functions, including cell division. Using the fluorescence in situ hybridization method, the gene encoding catalytic subunit-of PP2A alpha (locus symbol Ppp2ca) was assigned to rat chromosome 10 at a band of q22. The present study provides new evidence for the conserved syntenic association between human chromosome 5q23-q31 and the rat chromosome 10q22.