Metabolic pathway visualization in living yeast by DNP-NMR

被引：80
|
作者：
Meier, Sebastian [1 ]
Karlsson, Magnus [2 ]
Jensen, Pernille R. [2 ]
Lerche, Mathilde H. [2 ]
Duus, Jens O. [1 ]
机构：
[1] Carlsberg Lab, DK-1799 Copenhagen, Denmark
[2] Albeda Res, DK-1799 Copenhagen, Denmark
来源：
MOLECULAR BIOSYSTEMS | 2011年 / 7卷 / 10期
关键词：
MAGNETIC-RESONANCE; IN-VIVO; FLUX ANALYSIS; KINETICS; GLYCOLYSIS; PHOSPHATES; TUMORS;
D O I：
10.1039/c1mb05202k
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Central carbon metabolism of living Saccharomyces cerevisiae is visualized by DNP-NMR. Experiments are conducted as real time assays that detect metabolic bottlenecks, pathway use, reversibility of reactions and reaction mechanisms in vivo with subsecond time resolution.
引用
收藏
页码：2834 / 2836
页数：3
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