Molecular characterization of recombinant phosphoenolpyruvate carboxylase from an extreme thermophile

被引：0
|
作者：
Nakamura, T
Izui, K
机构：
[1] Kyoto Univ, Grad Sch Sci, Sakyo Ku, Kyoto 60601, Japan
[2] Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kyoto 60601, Japan
来源：
ADVANCES IN CHEMICAL CONVERSIONS FOR MITIGATING CARBON DIOXIDE | 1998年 / 114卷
关键词：
D O I：
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中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Phosphoenolpyruvate carboxylase (PEPC) of Thermus sp., was characterized and its mechanism of stabilization was studied by means of site-directed mutagenesis. A divergent sequence at a Gly-rich region of Thermus PEPC was revealed to contribute to the activity at high temperature but not to the tolerance to the irreversible heat inactivation.
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页码：605 / 608
页数：4
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