Wortmannin inhibits store-mediated calcium entry and protein tyrosine phosphorylation in human platelets

被引：16
|
作者：
Jenner, S [1 ]
Farndale, RW [1 ]
Sage, SO [1 ]
机构：
[1] UNIV CAMBRIDGE,DEPT BIOCHEM,CAMBRIDGE CB2 1QW,ENGLAND
来源：
FEBS LETTERS | 1996年 / 381卷 / 03期
关键词：
thapsigargin; tyrosine phosphorylation; store-mediated calcium influx; Wortmannin; platelet;
D O I：
10.1016/0014-5793(96)00130-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of the WT on store-mediated Ca2+ entry and protein tyrosine phosphorylation were investigated in fura-2-loaded human platelets, Wortmannin (2 mu M) attenuated the rise in [Ca2+](i) caused by Ca2+ entry,while having no effect on the mobilisation of Ca2+ from internal stores. It also reduced store-depletion-evoked protein tyrosine phosphorylation. These findings demonstrate that WT is an inhibitor of tyrosine phosphorylation and store-mediated calcium entry and provide further evidence for the involvement of a tyrosine phosphorylation step in the link between Ca2+ store depletion and Ca2+ influx in human platelets.
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页码：249 / 251
页数：3
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