DNA extraction from archival Giemsa-stained bone-marrow slides: comparison of six rapid methods

被引：32
|
作者：
Vince, A
Poljak, M
Seme, K
机构：
[1] Med Fac Ljubljana, Inst Microbiol & Immunol, Ljubljana 1105, Slovenia
[2] Dr Fran Mihaljevic Univ, Hosp Infect Dis, Zagreb, Croatia
来源：
BRITISH JOURNAL OF HAEMATOLOGY | 1998年 / 101卷 / 02期
关键词：
Giemsa; bone marrow slides; DNA; PCR; amplification;
D O I：
10.1046/j.1365-2141.1998.00702.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The ability of six rapid DNA extraction procedures to provide DNA for the polymerase chain reaction from archival Giemsa-stained bone marrow slides was tested on 120 samples. Boiling in distilled water, freeze-thaw method. boiling in 10% Chelex-100 resin solution, proteinase K/Tween 20/NP-40 method coupled with simplified phenol/chloroform/isoamyl alcohol protocol or salting-out procedure using saturated NaCl and modification of commercial QIAamp procedure (Qiagen, Chatsworth, Calif.) gave DNA extraction efficiencies of 50%, 70%, 85%, 95%, 100% and 100%, respectively. Our results demonstrate that rough DNA extraction methods have decreased efficiencies compared to complete DNA extraction protocols and that the latter are required to ensure highly reproducible results from archival Giemsa-stained bone marrow slides.
引用
收藏
页码：349 / 351
页数：3
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