Dual histone methyl reader ZCWPW1 facilitates repair of meiotic double strand breaks in male mice

被引：25

作者：

Mahgoub, Mohamed ^{[1
]}

Paiano, Jacob ^{[2
,3
]}

Bruno, Melania ^{[1
]}

Wu, Wei ^{[2
]}

Pathuri, Sarath ^{[4
]}

Zhang, Xing ^{[4
]}

Ralls, Sherry ^{[1
]}

Cheng, Xiaodong ^{[4
]}

Nussenzweig, Andre ^{[2
]}

Macfarlan, Todd S. ^{[1
]}

机构：

[1] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Bethesda, MD 20892 USA

[2] NCI, Lab Genome Integr, NIH, Bethesda, MD 20892 USA

[3] Univ Penn, Immunol Grad Grp, Philadelphia, PA 19104 USA

[4] Univ Texas MD Anderson Canc Ctr, Dept Epigenet & Mol Carcinogenesis, Houston, TX 77030 USA

来源：

ELIFE | 2020年 / 9卷

基金：

美国国家卫生研究院;

关键词：

SINGLE-NUCLEOTIDE POLYMORPHISMS; PRDM9 MEISETZ GENE; RECOMBINATION HOTSPOTS; METHYLTRANSFERASE ACTIVITY; CW DOMAIN; BINDING; ALIGNMENT; REVEALS; NUCLEOSOMES; CROSSOVERS;

D O I：

10.7554/eLife.53360

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Meiotic crossovers result from homology-directed repair of DNA double-strand breaks (DSBs). Unlike yeast and plants, where DSBs are generated near gene promoters, in many vertebrates DSBs are enriched at hotspots determined by the DNA binding activity of the rapidly evolving zinc finger array of PRDM9 (PR domain zinc finger protein 9). PRDM9 subsequently catalyzes tri-methylation of lysine 4 and lysine 36 of Histone H3 in nearby nucleosomes. Here, we identify the dual histone methylation reader ZCWPW1, which is tightly co-expressed during spermatogenesis with Prdm9, as an essential meiotic recombination factor required for efficient repair of PRDM9-dependent DSBs and for pairing of homologous chromosomes in male mice. In sum, our results indicate that the evolution of a dual histone methylation writer/reader (PRDM9/ZCWPW1) system in vertebrates remodeled genetic recombination hotspot selection from an ancestral static pattern near genes towards a flexible pattern controlled by the rapidly evolving DNA binding activity of PRDM9.

引用

页数：25

共 27 条

[21] E2F1 acetylation directs p300/CBP-mediated histone acetylation at DNA double-strand breaks to facilitate repair
Manickavinayaham, Swarnalatha
Velez-Cruz, Renier
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NATURE COMMUNICATIONS, 2019, 10 (1)
[22] E2F1 acetylation directs p300/CBP-mediated histone acetylation at DNA double-strand breaks to facilitate repair
Swarnalatha Manickavinayaham
Renier Vélez-Cruz
Anup K. Biswas
Ella Bedford
Brianna J. Klein
Tatiana G. Kutateladze
Bin Liu
Mark T. Bedford
David G. Johnson
Nature Communications, 10
[23] Histone deacetylase inhibitors promote persistent binding of PARP-1 to DNA double strand breaks in chromatin, thus decreasing repair via non-homologous end joining
Robert, Carine
Nagaria, Pratik K.
Gojo, Ivana
Rassooi, Feyruz
CANCER RESEARCH, 2015, 75
[24] Genetic Interactions of Histone Modification Machinery Set1 and PAF1C with the Recombination Complex Rec114-Mer2-Mei4 in the Formation of Meiotic DNA Double-Strand Breaks
Zhang, Ying
Suzuki, Takuya
Li, Ke
Gothwal, Santosh K.
Shinohara, Miki
Shinohara, Akira
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (08)
[25] The RSF1 Histone-Remodelling Factor Facilitates DNA Double Strand Break Repair by Recruiting Centromeric and Fanconi Anaemia Proteins (vol 12, e1001856, 2014)
Pessina, Fabio
Lowndes, Noel F.
PLOS BIOLOGY, 2017, 15 (02):
[26] The DNA helicase FANCJ (BRIP1) functions in double strand break repair processing, but not crossover formation during prophase I of meiosis in male mice
Horan, Tegan S.
Ascencao, Carolline F. R.
Mellor, Christopher A.
Wang, Meng
Smolka, Marcus B.
Cohen, Paula E.
PLOS GENETICS, 2024, 20 (02):
[27] Expression of Concern: The RSF1 Histone-Remodelling Factor Facilitates DNA Double-Strand Break Repair by Recruiting Centromeric and Fanconi Anaemia Proteins (Expression of Concern of Vol 12, art no E1001856, 2014)
Pessina, F.
Lowndes, N. F.
PLOS BIOLOGY, 2024, 22 (05)

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