The role of extracellular matrix, integrins, and cytoskeleton in mechanotransduction of centrifugal loading

被引：23
|
作者：
Li, Juan [1 ,2 ]
Zhao, Zhihe [1 ,2 ]
Wang, Jun [1 ]
Chen, Guoping [3 ]
Yang, Jingyuan [4 ]
Luo, Songjiao [1 ]
机构：
[1] Sichuan Univ, W China Coll Stomatol, Dept Orthodont, Chengdu 610041, Peoples R China
[2] Sichuan Univ, State Key Lab Oral Biomed Engn, Chengdu 610064, Peoples R China
[3] Zhejiang Univ, Sir Run Run Shao Hosp, Dept Dent, Hangzhou 310027, Peoples R China
[4] Wenzhou Med Coll, Hosp Stomatol, Wenzhou, Peoples R China
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 2008年 / 309卷 / 1-2期
关键词：
mechanotransduction; centrifugal force; ECM; integrin; F-actin; alpha-actin; fibronection; osteopontin; Collagen I;
D O I：
10.1007/s11010-007-9641-0
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The study was aimed to investigate the role of the "extracellular matrix (ECM)-integrins-cytoskeleton" signal pathway in mechanotransduction of centrifugal loading. MG-63 osteoblasts were exposed to centrifugal loading at 209xg for 10 min. Uncentrifuged cells and centrifuged cells that have been trypsinized and suspended in liquors were designed as control. The changes in F-actin and alpha-actin cytoskeleton, gene transcription of ECM components, and integrins expression were analyzed by LSCM, Real-Time RT-PCR and FCM, respectively. A temporary and fast reversible change was observed in F-actin and alpha-actin cytoskeleton. And the change was paralleled with the fast autoregulation in gene transcription of ECM components of fibronection, osteopontin and Collagen I, and integrins expression of both alpha 2 and beta 1 subunits. The result suggested that cytoskeleton was a possible mechanical sensor to centrifugal stimuli, and the cytoskeleton regulation to centrifugal loading was in an ECM-dependent and integrin-mediated manner.
引用
收藏
页码：41 / 48
页数：8
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