Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth

被引：4
|
作者：
Zhu, Hailin [1 ,2 ]
Yang, Jinliang [1 ]
Wei, Yuquan [1 ]
Chen, Harry Huimin [1 ,2 ]
机构：
[1] Sichuan Univ, State Key Lab Biotherapy, Chengdu 610041, Sichuan, Peoples R China
[2] StemEasy Biotech Ltd, Jiang Yin 214400, Jiangsu, Peoples R China
来源：
STEM CELLS INTERNATIONAL | 2015年 / 2015卷
关键词：
PROLONGED UNDIFFERENTIATED GROWTH; RECOMBINANT VITRONECTIN; HUMAN FEEDERS; SELF-RENEWAL; DERIVATION; LINES;
D O I：
10.1155/2015/621057
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells. For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components. Extracellular matrix (ECM) derived from feeder cells is the most natural way to provide xeno-free substrates for hESC growth. In this study, we optimized the step-by-step procedure for ECM processing to develop a xeno-free ECM that supports the growth of undifferentiated hESCs. In addition, this newly developed xeno-free substrate can be stored at 4 degrees C and is ready to use upon request, which serves as an easier way to amplify hESCs for clinical applications.
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页数：8
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