Differentiation of environmental and clinical isolates of Vibrio mimicus from Vibrio cholerae by multilocus enzyme electrophoresis

被引：23
|
作者：
Vieira, VV
Teixeira, LFM
Vicente, CP
Momen, H
Salles, CA
机构：
[1] Inst Oswaldo Cruz, Dept Biochem & Mol Biol, BR-20001 Rio De Janeiro, Brazil
[2] Inst Oswaldo Cruz, Dept Genet, BR-20001 Rio De Janeiro, Brazil
[3] Univ Fed Ouro Preto, Dept Clin Anal, Ouro Preto, Minas Gerais, Brazil
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 05期
关键词：
D O I：
10.1128/AEM.67.5.2360-2364.2001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In this study, we demonstrated that analyzed strains of Vibrio mimicus and Vibrio cholerae could be separated in two groups by using multilocus enzyme electrophoresis (MEE) data from 14 loci. We also showed that the combination of four enzymatic loci enables us to differentiate these two species. Our results showed that the ribosomal intergenic spacer regions PCR-mediated identification system failed, in some cases, to differentiate between V. mimicus and V. cholerae. On the other hand, MEE proved to be a powerful molecular tool for the discrimination of these hvo species even when atypical strains were analyzed.
引用
收藏
页码：2360 / 2364
页数：5
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