Agrobacterium bioassay strain for ultrasensitive detection of N-acylhomoserine lactone-type quorum-sensing molecules:: Detection of autoinducers in Mesorhizobium huakuii

被引：177
|
作者：
Zhu, J [1 ]
Chai, YR
Zhong, ZT
Li, SP
Winans, SC
机构：
[1] Nanjing Agr Univ, Dept Microbiol, MOA Key Lab Microbiol Engn Agr Environm, Nanjing, Peoples R China
[2] Cornell Univ, Dept Microbiol, Ithaca, NY 14853 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 11期
关键词：
D O I：
10.1128/AEM.69.11.6949-6953.2003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An ultrasensitive bioassay system for the detection of N-acylhomoserine lactones (AHLs) was constructed in Agrobacterium tumefaciens by using the T7 expression system to overproduce the AHL receptor TraR. This strain detected many diverse AHLs, some at extremely low concentrations. We used this strain to detect for the first time AHLs made by Mesorhizobium huakuii, which symbiotically fixes nitrogen in association with the legume Astragalus sinicus, a source of green manure throughout eastern Asia.
引用
收藏
页码：6949 / 6953
页数：5
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