Inducible cyclooxygenase expression in canine basilar artery after experimental subarachnoid hemorrhage

Background and Purpose-Inducible cyclooxygenase (COX-2) has been found to play a pathological role in cerebral insult. We investigated the expression of COX-2 in the basilar artery after experimental subarachnoid hemorrhage (SAH). Methods-In a canine "two-hemorrhage" model of SAH, the basilar arteries were obtained on day 2 after a cisternal injection of autologous blood or on days 4, 6, 7, or 9 after the second injection. Basilar arteries also were obtained 12 hours after intracisternal injection a cytokine: interleukin (IL)-1 beta (0.03 mu g), IL-6 (3 mu g), or IL-8 (10 mu g). Western blotting with a polyclonal anti-COX-2 antibody was performed in these arteries. Results-COX-2 protein was not demonstrated in the basilar artery in control animals without SAH. However, it was expressed in the basilar artery on days 2, 4, 6, and 7 after blood injection but not on day 9, Intracisternal injection of IL-1 beta, IL-6, or IL-8 also induced COX-2 in the basilar artery. Conclusions-COX-2 expression was detected in basilar arterial tissue in both acute and chronic stages after SAH, Elevation of inflammatory cytokines after SAH may be involved in the induction of COX-2, which may produce sufficient quantities of eicosanoids to affect hemodynamics after SAH.