Expression profile of sonic hedgehog signaling-related molecules in basal cell carcinoma

被引:20
|
作者
Kim, Hye Sung [1 ]
Kim, Young Sil [1 ]
Lee, Chul [2 ]
Shin, Myung Soo [3 ]
Kim, Jae Wang [4 ]
Jang, Bo Gun [1 ]
机构
[1] Jeju Natl Univ, Dept Pathol, Coll Med, Jeju, South Korea
[2] Seoul Natl Univ, Dept Pathol, Coll Med, Seoul, South Korea
[3] Jeju Natl Univ, Dept Plast Surg, Coll Med, Jeju, South Korea
[4] Jeju Natl Univ, Dept Dermatol, Coll Med, Jeju, South Korea
来源
PLOS ONE | 2019年 / 14卷 / 11期
关键词
HAIR FOLLICLE; STEM-CELLS; HUMAN HOMOLOG; GLI1; ACTIVATION; MUTATIONS; PROTEIN; LGR6;
D O I
10.1371/journal.pone.0225511
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Basal cell carcinoma (BCC) is the most common human cancer, characterized by aberrant activation of the hedgehog (HH) signaling pathway resulting from mutations in the patched 1 (PTCH1) or smoothened (SMO) genes. In the present study, to uncover the expression profile of HH signaling-related molecules, we thoroughly examined the mRNA and protein expression levels of six molecules including GLI1, GLI2, PTCH1, PTCH2, SHH, and SMO in BCC and various other cutaneous tumors. Real-time PCR analysis demonstrated that BCC showed remarkably enhanced mRNA expression of all HH molecules, except SMO compared to other skin tumors. However, immunohistochemical analysis revealed that only GLI1 protein was specifically upregulated in BCC, while the other HH-related proteins did not show any significant differences between the tumors. Notably, other skin malignancies such as squamous cell carcinoma, sebaceous carcinoma, and malignant melanoma showed no GLI1 expression and there was no difference in GLI1 expression between the BCC subtypes. In addition, GLI1 and GLI2 expression were strongly associated with the hair follicle stem cell markers, LGR4 and LGR5, which are known target genes of the Wnt pathway. Our results suggest that GLI1 has the potential to be a diagnostically useful marker for differentiating BCC from other skin malignancies and an interaction between the HH and Wnt signaling pathways may be involved in the development of BCCs.
引用
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页数:12
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