Error-Prone Replication through UV Lesions by DNA Polymerase θ Protects against Skin Cancers

被引：86
|
作者：
Yoon, Jung-Hoon [1 ]
McArthur, Mark J. [2 ]
Park, Jeseong [1 ]
Basu, Debashree [1 ]
Wakamiya, Maki [1 ]
Prakash, Louise [1 ]
Prakash, Satya [1 ]
机构：
[1] Univ Texas Med Branch, Dept Biochem & Mol Biol, 301 Univ Blvd, Galveston, TX 77555 USA
[2] Univ Texas MD Anderson Canc Ctr, Dept Vet Med & Surg, Houston, TX 77030 USA
来源：
CELL | 2019年 / 176卷 / 06期
关键词：
POL-THETA; MUTATIONAL LANDSCAPE; GENOMIC INSTABILITY; PYRIMIDINE DIMERS; THYMINE GLYCOL; COMET ASSAY; P53; GENE; BYPASS; ETA; DAMAGE;
D O I：
10.1016/j.cell.2019.01.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cancers from sun-exposed skin accumulate "driver'' mutations, causally implicated in oncogenesis. Because errors incorporated during translesion synthesis (TLS) opposite UV lesions would generate these mutations, TLS mechanisms are presumed to underlie cancer development. To address the role of TLS in skin cancer formation, we determined which DNA polymerase is responsible for generating UV mutations, analyzed the relative contributions of error-free TLS by Pol eta and error-prone TLS by Pol theta to the replication of UV-damaged DNA and to genome stability, and examined the incidence of UV-induced skin cancers in Pol theta(-/-), Pol eta(-/-), and Pol theta(-/-) Pol eta(-/-) mice. Our findings that the incidence of skin cancers rises in Pol theta(-/-) mice and is further exacerbated in Pol theta(-/-) Pol eta(-/-) mice compared with Pol eta(-/-) mice support the conclusion that error-prone TLS by Pol theta provides a safeguard against tumorigenesis and suggest that cancer formation can ensue in the absence of somatic point mutations.
引用
收藏
页码：1295 / +
页数：30
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