Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions

被引：18
|
作者：
Lin, Yongshun [1 ]
Zou, Jizhong [1 ]
机构：
[1] NHLBI, iPSC Core, NIH, Bethesda, MD 20892 USA
来源：
STAR PROTOCOLS | 2020年 / 1卷 / 01期
关键词：
D O I：
10.1016/j.xpro.2020.100015
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014; Lian et al., 2012; Lin et al., 2017). To help researchers adapt to our simple and costeffective method, we provide step-by-step protocols for hPSC-cardiomyocyte differentiation, including hPSC culture, cardiomyocyte differentiation, cardiomyocyte passaging, and cryopreservation.For complete details on the use and execution of this protocol, please refer to Lin et al. (2017).
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页数：15
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