A 1-Mb BAC/PAC-Based physical map of the autosomal recessive polycystic kidney disease gene (PKHD1) region on chromosome 6

被引：14
|
作者：
Park, JH
Dixit, MP
Onuchic, LF
Wu, GQ
Goncharuk, AN
Kneitz, S
Santarina, LB
Hayashi, T
Avner, ED
Guay-Woodford, L
Zerres, K
Germino, GG
Somlo, S
机构：
[1] Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA
[2] Albert Einstein Coll Med, Dept Mol Genet, Bronx, NY 10461 USA
[3] Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA
[4] Case Western Reserve Univ, Rainbow Babies & Childrens Hosp, Dept Pediat, Cleveland, OH 44106 USA
[5] Univ Alabama, Dept Pediat, Birmingham, AL 35294 USA
[6] Univ Alabama, Dept Med, Birmingham, AL 35294 USA
[7] Univ Bonn, Inst Human Genet, D-5111 Bonn, Germany
来源：
GENOMICS | 1999年 / 57卷 / 02期
关键词：
D O I：
10.1006/geno.1999.5777
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The PKHD1 (polycystic kidney and hepatic disease 1) gene responsible for autosomal recessive polycystic kidney disease has been mapped to 6p21.1-p12 to an similar to 1-cM interval flanked by the markers D6S1714/D6S243 and D6S1024. We have developed a sequence-ready BAG/PAC-based contig map of this region as the next step for the positional cloning of PKHD1. This contig comprising 52 clones spanning similar to 1 Mb was established by content mapping of 44 BAG/PAC-end-derived STSs, 3 known genetic markers, 5 YAC-end-derived STSs, 3 random STSs, 1 previously mapped gene, and 1 EST. The average depth per marker is 6.3 clones, and the average STS density is 20 kb. The genomic clone overlaps were confirmed by restriction fragment fingerprint analysis. A high-resolution BAC/PAC-based contig map is essential to the ultimate goal of identifying the PKHD1 gene. (C) 1999 Academic Press.
引用
收藏
页码：249 / 255
页数：7
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