Molecular Epidemiology of Mycobacterium avium subsp paratuberculosis in a Longitudinal Study of Three Dairy Herds

被引:54
|
作者
Pradhan, Abani K. [1 ]
Mitchell, Rebecca M. [1 ]
Kramer, Aagje J. [2 ]
Zurakowski, Michael J. [1 ]
Fyock, Terry L. [3 ]
Whitlock, Robert H. [3 ]
Smith, Julia M. [4 ]
Hovingh, Ernest [5 ]
Van Kessel, Jo Ann S. [6 ]
Karns, Jeffrey S. [6 ]
Schukken, Ynte H. [1 ]
机构
[1] Cornell Univ, Qual Milk Prod Serv, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA
[2] Univ Utrecht, Fac Vet Med, Utrecht, Netherlands
[3] Univ Penn, New Bolton Ctr, Dept Clin Studies, Kennett Sq, PA 19348 USA
[4] Univ Vermont, Dept Anim Sci, Burlington, VT 05405 USA
[5] Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA
[6] ARS, Environm Microbial & Food Safety Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA
关键词
JOHNES-DISEASE STATUS; UNITED-STATES; GENETIC DIVERSITY; INFECTION STATUS; CATTLE; COWS; SEQUENCE; CULTURE; ASSOCIATION; PREVALENCE;
D O I
10.1128/JCM.01107-10
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The objective of this study was to evaluate whether cows that were low shedders of Mycobacterium avium subsp. paratuberculosis were passively shedding or truly infected with M. avium subsp. paratuberculosis. We also investigated whether it is possible that these M. avium subsp. paratuberculosis-infected animals could have been infected as adults by contemporary high-shedding animals (supershedders). The M. avium subsp. paratuberculosis isolates were obtained from a longitudinal study of three dairy herds in the northeastern United States. Isolates were selected from fecal samples and tissues at slaughter from all animals that were culture positive at the same time that supershedders were present in the herds. Shedding levels (CFU of M. avium subsp. paratuberculosis/g of feces) for the animals at each culture-positive occasion were determined. Using a multilocus short-sequence-repeat technique, we found 15 different strains of M. avium subsp. paratuberculosis from a total of 142 isolates analyzed. Results indicated herd-specific infection patterns; there was a clonal infection in herd C, with 89% of isolates from animals sharing the same strain, whereas herds A and B showed several different strains infecting the animals at the same time. Tissues from 80% of cows with at least one positive fecal culture (other than supershedders) were culture positive, indicating a true M. avium subsp. paratuberculosis infection. The results of M. avium subsp. paratuberculosis strain typing and observed shedding levels showed that at least 50% of low shedders have the same strain as that of a contemporary supershedder. Results of this study suggest that in a dairy herd, more of the low-shedding cows are truly infected with M. avium subsp. paratuberculosis than are passively shedding M. avium subsp. paratuberculosis. The sharing of strains between low shedders and the contemporary supershedders suggests that low shedders may have been infected by environmental exposure of M. avium subsp. paratuberculosis.
引用
收藏
页码:893 / 901
页数:9
相关论文
共 50 条
  • [21] Genotyping methods and molecular epidemiology of Mycobacterium avium subsp. paratuberculosis (MAP)
    Fawzy, Ahmad
    Zschoeck, Michael
    Ewers, Christa
    Eisenberg, Tobias
    [J]. INTERNATIONAL JOURNAL OF VETERINARY SCIENCE AND MEDICINE, 2018, 6 (02) : 258 - 264
  • [22] The Effect of Mycobacterium avium subsp. Paratuberculosis Infection on the Productivity of Cows in Two Dairy Herds with a Low Seroprevalence of Paratuberculosis
    Wiszniewska-Laszczych, Agnieszka
    Liedtke, Katarzyna G.
    Szteyn, Joanna M.
    Lachowicz, Tomasz
    [J]. ANIMALS, 2020, 10 (03):
  • [23] Longitudinal study of the distribution of Mycobacterium avium subsp paratuberculosis in the environment of dairy herds in the Michigan Johne's disease control demonstration herd project
    Pillars, Roxanne B.
    Grooms, Daniel L.
    Kaneene, John B.
    [J]. CANADIAN VETERINARY JOURNAL-REVUE VETERINAIRE CANADIENNE, 2009, 50 (10): : 1039 - 1046
  • [24] Herd characteristics and management practices associated with seroprevalence of Mycobacterium avium subsp paratuberculosis infection in dairy herds
    Tavornpanich, Saraya
    Johnson, Wesley O.
    Anderson, Randall J.
    Gardner, Ian A.
    [J]. AMERICAN JOURNAL OF VETERINARY RESEARCH, 2008, 69 (07) : 904 - 911
  • [25] Interpretation of the serum ELISA for detection of Mycobacterium avium subsp paratuberculosis fecal shedding in dairy cattle herds
    Wells, SJ
    Godden, S
    Whitlock, RH
    Collins, J
    [J]. PROCEEDINGS OF THE SEVENTH INTERNATIONAL COLLOQUIUM ON PARATUBERCULOSIS, 2003, : 406 - 407
  • [26] Genomic epidemiology of Mycobacterium avium subsp. paratuberculosis isolates from Canadian dairy herds provides evidence for multiple infection events
    Byrne, Alexander
    Ollier, Severine
    Tahlan, Kapil
    Biet, Franck
    Bissonnette, Nathalie
    [J]. FRONTIERS IN GENETICS, 2023, 14
  • [27] Fecal shedding of Mycobacterium avium subsp paratuberculosis by dairy cows
    Crossley, BM
    Zagmutt-Vergara, FJ
    Fyock, TL
    Whitlock, RH
    Gardner, IA
    [J]. VETERINARY MICROBIOLOGY, 2005, 107 (3-4) : 257 - 263
  • [28] Prevalence of Mycobacterium avium subsp paratuberculosis (Map) in the dairy herd
    Buckley, J
    [J]. IRISH VETERINARY JOURNAL, 2004, 57 (07) : 397 - 397
  • [29] Observed management practices in relation to the risk of infection with paratuberculosis and to the spread of Mycobacterium avium subsp paratuberculosis in Swiss dairy and beef herds
    Kuenzler, Rahel
    Torgerson, Paul
    Keller, Selina
    Wittenbrink, Max
    Stephan, Roger
    Knubben-Schweizer, Gabriela
    Berchtold, Beat
    Meylan, Mireille
    [J]. BMC VETERINARY RESEARCH, 2014, 10
  • [30] Prevalence of Mycobacterium avium ssp paratuberculosis infections in Canadian dairy herds
    Corbett, Caroline S.
    Naqvi, S. Ali
    Bauman, Cathy A.
    De Buck, Jeroen
    Orsel, Karin
    Uehlinger, Fabienne
    Kelton, David F.
    Barkema, Herman W.
    [J]. JOURNAL OF DAIRY SCIENCE, 2018, 101 (12) : 11218 - 11228