Separation and detection of erythropoietin by CE and CE-MS

被引：15

作者：

Yu, B ^{[1
]}

Cong, HL ^{[1
]}

Liu, HW ^{[1
]}

Li, YZ ^{[1
]}

Liu, F ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2005年 / 24卷 / 04期

基金：

中国国家自然科学基金;

关键词：

capillary electrophoresis; detection; erythropoietin; mass spectrometry; review;

D O I：

10.1016/j.trac.2004.11.020

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

For doping control, the standard method of testing recombinant human erythropoietin (rhEPO) is a combination of a blood test and a urine test. The blood test is based primarily on changes in blood parameters, and the urine test employs an immunoblotting assay that includes one isoelectric focusing (IEF) separation step, two blotting steps and luminescence detection, so the whole testing procedure is time-consuming and expensive. The present review summarizes the separation and the detection of rhEPO by capillary electrophoresis (CE) as well as by CE on-line coupled with electrospray ionization mass spectrometry (CE-ESI-MS). We demonstrate the usefulness of these approaches. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：350 / 357

页数：8

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