Basic aspects of absorption and fluorescence spectroscopy and resonance energy transfer methods

被引:11
|
作者
Shanker, Natasha [1 ]
Bane, Susan L. [1 ]
机构
[1] SUNY Binghamton, Dept Chem, Binghamton, NY 13902 USA
来源
BIOPHYSICAL TOOLS FOR BIOLOGISTS: VOL 1 IN VITRO TECHNIQUES | 2008年 / 84卷
关键词
D O I
10.1016/S0091-679X(07)84008-8
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Absorption and fluorescence spectroscopy are the heart of life science laboratory techniques. These two spectroscopic tools are sensitive, are easy to use, and can provide a wide range of information. The goal of this chapter is to familiarize the reader with some basic principles and applications of absorption and steady-state fluorescence spectroscopy. Selected applications are illustrated with examples.
引用
收藏
页码:213 / 242
页数:30
相关论文
共 50 条
  • [41] ABSORPTION-SPECTROSCOPY USING MICRODROPLET RESONANCE FLUORESCENCE INTENSITIES
    EVERSOLE, JD
    LIN, HB
    MERRITT, CD
    CAMPILLO, AJ
    APPLIED SPECTROSCOPY, 1994, 48 (03) : 373 - 381
  • [42] Protein Labeling Facilitates the Understanding of Protein Corona Formation via Fluorescence Resonance Energy Transfer and Fluorescence Correlation Spectroscopy
    Gao, Lian-Xun
    Hao, Hao
    Yu, Ying-Qi
    Chen, Ji-Lei
    Chen, Wen-Qi
    Gong, Zuo-Dong
    Liu, Yi
    Jiang, Feng-Lei
    LANGMUIR, 2023, 39 (43) : 15275 - 15284
  • [43] Two new concepts to measure fluorescence resonance energy transfer via fluorescence correlation spectroscopy: Theory and experimental realizations
    Widengren, J
    Schweinberger, E
    Berger, S
    Seidel, CAM
    JOURNAL OF PHYSICAL CHEMISTRY A, 2001, 105 (28): : 6851 - 6866
  • [44] Fluorescence resonance energy transfer-based analytical methods for determination of food contaminants: A review
    Liang, Yuan
    Gong, Yiyao
    Yu, Yifan
    Jiang, Qiuyan
    Zhao, Jingqi
    Zou, Haoyang
    Zhang, Jie
    FOOD CONTROL, 2023, 152
  • [46] Fluorescence resonance energy transfer imaging via fluorescence polarization measurement
    Cohen-Kashi, Meir
    Namer, Yaniv
    Deutsch, Mordechai
    JOURNAL OF BIOMEDICAL OPTICS, 2006, 11 (03)
  • [47] Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy
    Gordon, GW
    Berry, G
    Liang, XH
    Levine, B
    Herman, B
    BIOPHYSICAL JOURNAL, 1998, 74 (05) : 2702 - 2713
  • [48] BASIC PRINCIPLES AND TECHNICAL ASPECTS OF MAGNETIC-RESONANCE SPECTROSCOPY IN PATIENTS
    GUCKEL, F
    BACHERTBAUMANN, P
    SEMMLER, W
    RADIOLOGE, 1989, 29 (07): : 322 - 329
  • [49] THE CURRENT STATUS OF MAGNETIC-RESONANCE SPECTROSCOPY - BASIC AND CLINICAL ASPECTS
    CHAN, L
    WESTERN JOURNAL OF MEDICINE, 1985, 143 (06): : 773 - 781
  • [50] Modulating fluorescence resonance energy transfer in conjugated liposomes
    Li, Xuelian
    McCarroll, Matthew
    Kohli, Punit
    LANGMUIR, 2006, 22 (21) : 8615 - 8617