Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching

被引：21
|
作者：
Lenn, Tchern [1 ]
Gkekas, Christos N. [2 ,3 ]
Bernard, Laurent [2 ]
Engl, Christoph [1 ,2 ]
Jovanovic, Goran [1 ]
Buck, Martin [1 ]
Ying, Liming [2 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Div Biol, London SW7 2AZ, England
[2] Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, London SW7 2AZ, England
[3] Univ London Imperial Coll Sci Technol & Med, Ctr Bioinformat, Div Mol Biosci, London SW7 2AZ, England
来源：
CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 01期
基金：
英国生物技术与生命科学研究理事会;
关键词：
ESCHERICHIA-COLI; IN-VIVO; PROTEIN COMPLEXES; SPECTROSCOPY; REPRESSION; MICROSCOPY; MEMBRANES; DYNAMICS; RECEPTOR; RING;
D O I：
10.1039/c0cc01707h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.
引用
收藏
页码：400 / 402
页数：3
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