Wnt2 protein plays a role in the progression of pancreatic cancer promoted by pancreatic stellate cells

被引：21

作者：

Xu, Yong ^{[1
]}

Li, Hua ^{[2
]}

Huang, Chongbiao ^{[3
]}

Zhao, Tiansuo ^{[4
]}

Zhang, Huan ^{[4
]}

Zheng, Chen ^{[4
]}

Ren, He ^{[4
]}

Hao, Jihui ^{[4
,5
]}

机构：

[1] Tianjin Med Univ Canc Inst & Hosp, Dept Diagnost & Therapeut Ultrasonog, Natl Clin Res Ctr Canc, Key Lab Canc Prevent & Therapy, Tianjin 300060, Peoples R China

[2] Tianjin Med Univ Canc Inst & Hosp, Dept Endoscopy, Natl Clin Res Ctr Canc, Key Lab Canc Prevent & Therapy, Tianjin 300060, Peoples R China

[3] Tianjin Med Univ Canc Inst & Hosp, Dept Senior Wards, Natl Clin Res Ctr Canc, Key Lab Canc Prevent & Therapy, Tianjin 300060, Peoples R China

[4] Tianjin Med Univ Canc Inst & Hosp, Dept Pancreat Canc, Natl Clin Res Ctr Canc, Key Lab Canc Prevent & Therapy, Tianjin 300060, Peoples R China

[5] Tianjin Med Univ Canc Inst & Hosp, Dept Pancreat Canc, Tianjin 300060, Peoples R China

来源：

MEDICAL ONCOLOGY | 2015年 / 32卷 / 04期

关键词：

Pancreatic cancer; Stellate cells; Wnt signal pathway; Wnt2; Epithelial-mesenchymal transition; BETA-CATENIN; ACTIVATION; CULTURE;

D O I：

10.1007/s12032-015-0513-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This study aimed to investigate the expression of Wnt2 protein in pancreatic cancer tissues and pancreatic stellate cells (PSCs), and determine its effect on the biological functions of pancreatic cancer cells. Immunohistochemistry was used to study the expression pattern of Wnt2 in pancreatic cancer tissues. The relationship between Wnt2 protein expression level and patient prognosis was analyzed. PSCs were isolated and cultured. The expression of Wnt2 in activated PSCs was investigated using Western blot and immunofluorescence. We also analyzed the effect of Wnt2 recombinant protein and stellate cell culture supernatant on the Wnt/beta-catenin signaling pathway, as well as the effect of Wnt2 recombinant protein on the biological functions of pancreatic cancer cells. The expression of Wnt2 in interstitial cells of pancreatic cancer was correlated with the prognosis of pancreatic cancer. Wnt2 protein was expressed in activated PSCs. Both stellate cell culture supernatant and Wnt2 recombinant protein could activate the classic Wnt/beta-catenin signaling pathway. Wnt2 protein enhanced the migration, invasion, and metastasis of pancreatic cancer cells. These results suggested that Wnt2 protein secreted by PSCs promoted the progression of pancreatic cancer by activating the classic Wnt/beta-catenin signaling pathway.

引用

页数：8

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