An integrated microfluidic biochemical detection system with magnetic bead-based sampling and analysis capabilities

被引：6

作者：

Choi, JW ^{[1
]}

Oh, KW ^{[1
]}

Thomas, JH ^{[1
]}

Heineman, WR ^{[1
]}

Halsall, HB ^{[1
]}

Nevin, JH ^{[1
]}

Helmicki, AJ ^{[1
]}

Henderson, HT ^{[1
]}

Ahn, CH ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Elect & Comp Engn, Microsyst & Biomems Lab, Cincinnati, OH 45221 USA

来源：

14TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST | 2001年

关键词：

D O I：

10.1109/MEMSYS.2001.906574

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

This paper presents the development and characterization of an integrated microfluidic biochemical detection system for fast and low volume immunoassays using magnetic beads, which are used as both immobilization surfaces and bio-molecule carriers. Magnetic bead-based immunoassay, as a typical example of biochemical detection and analysis, has been successfully performed on the integrated microfluidic biochemical analysis system that includes a surface-mounted biofilter and immunosensor on a glass microfluidic motherboard. Total time required for full immunoassay was less than 20 minutes including sample incubation time and sample volume wasted was less than 50 mul during five repeated assays. Fast and low volume biochemical analysis has been successfully achieved with the developed biofilter and immunosensor, which is integrated to microfluidic system.

引用

页码：447 / 450

页数：4

共 50 条

[41] Fine-tuning of magnetic and microfluidic viscous forces for specific magnetic bead-based immunocomplex formation
Cornaglia, M.
Tekin, H. C.
Lehnert, T.
Gijs, M. A. M.
JOURNAL OF APPLIED PHYSICS, 2013, 114 (06)
[42] ROLLING CIRCLE AMPLIFICATION IN BEAD-BASED MICROFLUIDIC DEVICE WITH INTEGRATED PHOTODIODE FOR FLUORESCENCE SIGNAL TRANSDUCTION
Caneira, Catarina R. F.
Soares, Ruben R. G.
Nikolaidou, Katerina
Nilsson, Mats
Madaboosi, Narayanan
Chu, Virginia
Conde, Joao P.
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021), 2021, : 575 - 578
[43] A magnetic bead-based protein kinase assay with dual detection techniques
Zhou, Guangchang
Sylvester, Juliesta E.
Wu, Ding
Veach, Darren R.
Kron, Stephen J.
ANALYTICAL BIOCHEMISTRY, 2011, 408 (01) : 5 - 11
[44] Establishment of magnetic bead-based immunosensor for the detection of tetracycline residues in milk
Kang, Huaibin
Ma, Hongyan
Li, Fang
Xu, Baocheng
Luo, Denglin
Zhang, Ruihua
Zou, Liangliang
Shipin Kexue/Food Science, 2016, 37 (20): : 113 - 119
[45] Magnetic bead-based fluorometric detection of lectin-glycoprotein interactions
Rambihar, Christopher
Kerman, Kagan
TALANTA, 2010, 81 (4-5) : 1676 - 1680
[46] Magnetic bead-based label-free chemiluminescence detection of telomeres
Lu, JZ
Lau, CW
Kai, M
CHEMICAL COMMUNICATIONS, 2003, (23) : 2888 - 2889
[47] Magnetic bead-based detection of autoimmune responses using protein microarrays
Gantelius, Jesper
Hartmann, Michael
Schwenk, Jochen M.
Roeraade, Johan
Andersson-Svahn, Helene
Joos, Thomas O.
NEW BIOTECHNOLOGY, 2009, 26 (06) : 269 - 276
[48] Integrated electrokinetic magnetic bead-based electrochemical immunoassay on microfluidic chips for reliable control of permitted levels of zearalenone in infant foods
Hervas, Mirian
Lopez, Miguel A.
Escarpa, Alberto
ANALYST, 2011, 136 (10) : 2131 - 2138
[49] Integrated Analyses of Proteins and Their Glycans in a Magnetic Bead-Based Multiplex Assay Format
Li, Danni
Chiu, Hanching
Chen, Jing
Zhang, Hui
Chan, Daniel W.
CLINICAL CHEMISTRY, 2013, 59 (01) : 315 - 324
[50] A bead-based microfluidic approach to integrated single-cell gene expression analysis by quantitative RT-PCR
Sun, Hao
Olsen, Tim
Zhu, Jing
Tao, Jianguo
Ponnaiya, Brian
Amundson, Sally A.
Brenner, David J.
Lin, Qiao
RSC ADVANCES, 2015, 5 (07): : 4886 - 4893

← 1 2 3 4 5 →