Determination of the Maximum Length of DNA in a Polymer Based on Linear Poly(N,N-Dimethylacrylamide) Decoded with an Accuracy of 99% by Capillary Gel Electrophoresis with Laser-Induced Fluorescence

被引：2
|
作者：
Bocharova, D. V. [1 ]
Alekseev, Ya. I. [1 ,2 ]
Volkov, A. A. [3 ]
Lavrov, G. S. [1 ]
Plugov, A. G. [2 ]
Volkov, I. A. [2 ]
Chemigov, A. A. [2 ]
Bardin, B. V. [1 ]
Kurochkin, V. E. [1 ]
机构：
[1] Russian Acad Sci, Inst Analyt Instrumentat, St Petersburg 198095, Russia
[2] Ltd Liabil Co Syntol, Moscow 127434, Russia
[3] Russian Fed Res Inst Fisheries & Oceanog, Moscow 107140, Russia
来源：
JOURNAL OF ANALYTICAL CHEMISTRY | 2021年 / 76卷 / 12期
关键词：
DNA sequencing; capillary gel electrophoresis; linear poly(N; N-dimethylacrylamide); genetic analyzer; Nanophore; 05; GA; 3500xL; SEQUENCE;
D O I：
10.1134/S106193482110004X
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
DNA sequencing is an essential task of modern molecular genetic research. Its fundamental parameter is the accuracy of decoding. In this work, we determined the maximum lengths of DNA fragments, obtained by the Sanger method and sequenced with an accuracy of 99%, in a polymer based on linear poly(N,N-dimethylacrylamide) PDMA-6 for capillaries 50 cm long to the optical window of the detector of a Nanophore 05 domestic genetic analyzer and an imported GA 3500xL genetic analyzer. The results of DNA sequencing obtained by various analysis algorithms were compared. The parameters of the analysis algorithms were selected, ensuring best results of DNA sequencing in using the PDMA-6 polymer.
引用
收藏
页码：1408 / 1413
页数：6
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