In vitro plant regeneration of Melia azedarach L.:: shoot organogenesis from leaf explants

被引：0

作者：

Vila, SK ^{[1
]}

Gonzalez, AM ^{[1
]}

Rey, HY ^{[1
]}

Mroginski, LA ^{[1
]}

机构：

[1] Fac Ciencias Agr UNNE, Inst Bot Nordeste, RA-3400 Corrientes, Argentina

来源：

BIOLOGIA PLANTARUM | 2003年 / 47卷 / 01期

关键词：

auxin; cytokinin; gibberellic acid; leaf culture; rooting;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In vitro regeneration of Melia azedarach L. was studied. Shoots were regenerated from calli initiated from leaflets of in vitro growing plants. The best medium for establishment of cultures was Murashige and Skoog (MS) medium with 4.44 muM benzylaminopurine (BAP) + 0.46 muM kinetin (KIN) + 16.29 muM adenine sulphate (ADE). Regenerated shoots were multiplied in MS + 0.44 muM BAP + 0.37 muM KIN + 3.26 muM ADE. Maximal rooting of 89% was achieved by culture of regenerated shoots in MS + 12.26 muM indole-3-butyric acid for 3 d and subsequently in MS lacking growth regulators for 27 d. Rooted shoots were acclimatized and successfully transferred to soil.

引用

页码：13 / 19

页数：7

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