15N-Labeled ionic probes for bioanalytical mass spectrometry

被引：3
|
作者：
Ito, Fumihiro [1 ]
Ando, Shin [1 ]
Iuchi, Masato [1 ]
Nakamura, Tomoko [1 ]
Yorita, Satoko [1 ]
Yamaguchi, Kentaro [1 ]
机构：
[1] Tokushima Bunri Univ, Fac Pharmaceut Sci, Kagawa 7692193, Japan
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2010年 / 8卷 / 19期
关键词：
ISOBARIC TAGGING ITRAQ; CODED AFFINITY TAGS; ELECTROSPRAY-IONIZATION; BENZOPHENONE FRAGMENT; LIQUID-CHROMATOGRAPHY; PROTEOME ANALYSIS; BIOMOLECULES; QUANTITATION; ABUNDANCE; REAGENTS;
D O I：
10.1039/c003784b
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
An effective La-complex-based probe ionization method is reported. Novel stable isotopically labeled probes containing the N-15-labeled 2,6-bis(oxazolin-2-yl)pyridine (pybox) ligand, succinimide-tetramethylpybox (NHS-TM pybox), maleimide-tetramethylpybox (Mal-TM pybox), and 4-(tetramethylpybox)-butyl bromoacetate (BrAc-TMpybox), have been synthesized and their value in analyzing large complex molecules has been studied. The value of the N-15-labeled pybox-La complex in ionizing various compounds, including bioactive peptides by cold-spray ionization mass spectrometry is emphasized.
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页码：4408 / 4413
页数：6
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