Experimental transmission of chronic wasting disease agent from mule deer to cattle by the intracerebral route

被引:83
|
作者
Hamir, AN
Kunkle, RA
Cutlip, RC
Miller, JM
O'Rourke, KI
Williams, ES
Miller, MW
Stack, MJ
Chaplin, MJ
Richt, JA
机构
[1] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA
[2] USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA
[3] Univ Wyoming, Dept Vet Sci, Laramie, WY 82070 USA
[4] Colorado Div Wildlife, Wildlife Res Ctr, Ft Collins, CO 80526 USA
[5] Vet Serv Agcy, Weybridge KT15 3NB, Surrey, England
关键词
cattle; chronic wasting disease (CWD) of mule deer; prion disease;
D O I
10.1177/104063870501700313
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
This communication reports final observations on experimental transmission of chronic wasting disease (CWD) from mule deer to cattle by the intracerebral route. Thirteen calves were inoculated intracerebrally with brain suspension from mule deer naturally affected with CWD. Three other calves were kept as uninoculated controls. The experiment was terminated 6 years after inoculation. During that time, abnormal prion protein (PrPres) was demonstrated in the central nervous system (CNS) of 5 cattle by both immunohistochemistry and Western blot. However, microscopic lesions suggestive of spongiform encephalopathy (SE) in the brains of these PrPres-positive animals were subtle in 3 cases and absent in 2 cases. Analysis of the gene encoding bovine PRNP revealed homozygosity for alleles encoding 6 octapeptide repeats, serine (S) at codon 46, and S at codon 146 in all samples. Findings of this study show that although PrPres amplification occurred after direct inoculation into the brain, none of the affected animals had classic histopathologic lesions of SE. Furthermore, only 38% of the inoculated cattle demonstrated amplification of PrPres. Although intracerebral inoculation is an unnatural route of exposure, this experiment shows that CWD transmission in cattle Could have long incubation periods (up to 5 years). This finding suggests that oral exposure of cattle to CWD agent, a more natural potential route of exposure, would require not only a much larger dose of inoculum but also may not result in amplification of PrPres within CNS tissues during the normal lifespan of cattle.
引用
收藏
页码:276 / 281
页数:6
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