A novel putative 2-oxoglutarate-dependent dioxygenase gene (BoaAOP-like) regulates aliphatic glucosinolate biosynthesis in Chinese kale

被引：4

作者：

Zheng, Hao ^{[1
]}

Wang, Yating ^{[1
]}

Li, Xiangxiang ^{[1
]}

Huang, Wenli ^{[1
]}

Miao, Huiying ^{[2
]}

Li, Huanxiu ^{[1
]}

Wang, Qiaomei ^{[2
]}

Sun, Bo ^{[1
]}

Zhang, Fen ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Hort, 211 Huimin Rd, Chengdu 611130, Sichuan, Peoples R China

[2] Zhejiang Univ, Dept Hort Dev & Qual Improvement, Key Lab Hort Plant Growth, Minist Agr, Hangzhou 310058, Peoples R China

来源：

SCIENTIA HORTICULTURAE | 2022年 / 297卷

基金：

中国国家自然科学基金;

关键词：

Chinese kale (Brassica oleracea var. alboglabra); Aliphatic glucosinolates; BoaAOP-like; Subcellular localization; Gene expression patterns; Transient gene overexpression; TRANSCRIPTION FACTORS; SIGNALING PATHWAYS; ARABIDOPSIS; METABOLISM; EXPRESSION; HEALTH; AOP2;

D O I：

10.1016/j.scienta.2022.110921

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Glucosinolates are important secondary metabolites in Brassica vegetables, and the side-chain modifications of aliphatic glucosinolates are regulated by 2-oxoglutarate-dependent dioxygenases (AOPs). However, how the side-chain modifications of aliphatic glucosinolates are regulated in Chinese kale remains unclear. Here, we show that the novel gene BoaAOP-like affects the biosynthesis of aliphatic glucosinolates in Chinese kale. BoaAOP-like was localized in the cytoplasm of Chinese kale. The expression levels of BoaAOP-like increased with plant growth; among different organs, BoaAOP-like was the highest in the roots. BoaAOP-like was introduced into Chinese kale using Agrobacterium-mediated transient overexpression system, of which the expression levels were 2.20- to 49.56-fold higher in transiently overexpressed plants than in wild-type plants; the content of total aliphatic glucosinolates in transiently overexpressed plants was up to 70.26-fold higher than in wild-type plants. The content of each aliphatic glucosinolate was significantly accumulated in BoaAOP-like transiently overexpressed plants, with the gluconapin content increasing the greatest when compared to the wild-type plants. The expression levels of aliphatic glucosinolate-related genes, especially MYB28 and AOP2.1 were significantly up-regulated in transiently overexpressed plants. Overall, our results indicated that BoaAOP-like plays a critical role in regulating the biosynthesis of aliphatic glucosinolate in Chinese kale.

引用

页数：9