A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU

被引：125
|
作者：
Higgins, P. G. [1 ]
Wisplinghoff, H. [1 ]
Krut, O. [1 ]
Seifert, H. [1 ]
机构：
[1] Univ Cologne, Inst Med Microbiol Immunol & Hyg, D-50935 Cologne, Germany
来源：
CLINICAL MICROBIOLOGY AND INFECTION | 2007年 / 13卷 / 12期
关键词：
Acinetobacter; genomic species; gyrB genes; identification; PCR; speciation;
D O I：
10.1111/j.1469-0691.2007.01819.x
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A new PCR-based method that exploits differences in gyrB gene sequences was developed to distinguish between Acinetobacter baumannii and Acinetobacter genomic sp. 13TU. Among 118 clinical and reference Acinetobacter strains, 102 of which were previously speciated by amplified rDNA restriction analysis as belonging to the Acinetobacter calcoaceticus-A. baumannii complex, the method correctly identified 31 A. baumannii and 54 Acinetobacter genomic sp. 13TU isolates to the species level. The method was rapid, specific and easy to interpret.
引用
收藏
页码：1199 / 1201
页数：4
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