Blood levels of D-amino acid oxidase vs. D-amino acids in reflecting cognitive aging

被引:73
|
作者
Lin, Chieh-Hsin [1 ,2 ,3 ]
Yang, Hui-Ting [4 ]
Chiu, Chih-Chiang [5 ]
Lane, Hsien-Yuan [2 ,6 ]
机构
[1] Chang Gung Univ, Dept Psychiat, Kaohsiung Chang Gung Mem Hosp, Coll Med, Kaohsiung, Taiwan
[2] China Med Univ, Grad Inst Biomed Sci, Taichung, Taiwan
[3] Cheng Shiu Univ, Ctr Gen Educ, Kaohsiung, Taiwan
[4] China Med Univ, Dept Nutr, Taichung, Taiwan
[5] Taipei City Psychiat Ctr, Dept Psychiat, Taipei, Taiwan
[6] China Med Univ Hosp, Dept Psychiat, Taichung, Taiwan
来源
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词
MINI-MENTAL-STATE; D-SERINE; ALZHEIMERS-DISEASE; CEREBROSPINAL-FLUID; GLUTAMATE; NEUROTRANSMISSION; SCHIZOPHRENIA; DEGENERATION; PROGRESSION; IMPAIRMENT;
D O I
10.1038/s41598-017-13951-7
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Feasible peripheral biomarker for Alzheimer's disease (AD) is lacking. Dysregulation of N-methyl-Daspartate (NMDA) receptor is implicated in the pathogenesis of AD. D-amino acid oxidase (DAO) and amino acids can regulate the NMDA receptor function. This study aimed to examine whether peripheral DAO and amino acids levels are characteristic of age-related cognitive decline. We enrolled 397 individuals (including amnestic mild cognitive impairment (MCI), mild AD, moderate to severe AD, and healthy elderly). DAO levels in the serum were measured using ELISA. Amino acids levels in serum were measured by high performance liquid chromatography. Severity of the cognitive deficits in subjects was assessed using Clinical Dementia Rating Scale (CDR). The DAO levels increased with the severity of the cognitive deficits. DAO levels were significantly associated with D-glutamate and D-serine levels. The Receiver Operating Characteristics analysis of DAO levels for AD patients vs. healthy controls determined the optimal cutoff value, 30.10, with high sensitivity (0.842) and specificity (0.889) (area under curve = 0.928). This is the first study indicating that the peripheral DAO levels may increase with age-related cognitive decline. The finding supports the hypofunction of NMDA receptor hypothesis in AD. Whether DAO could serve as a potential surrogate biomarker needs further studies.
引用
收藏
页数:10
相关论文
共 50 条
  • [21] Mutant D-amino acid oxidase with higher catalytic efficiency toward D-amino acids with bulky side chains
    Komarova, N. V.
    Golubev, I. V.
    Khoronenkova, S. V.
    Tishkov, V. I.
    RUSSIAN CHEMICAL BULLETIN, 2012, 61 (07) : 1489 - 1496
  • [22] Enzymatic conversion of unnatural amino acids by yeast D-amino acid oxidase
    Caligiuri, Antonio
    D'Arrigo, Paola
    Rosini, Elena
    Tessaro, Davide
    Molla, Gianluca
    Servi, Stefano
    Pollegioni, Loredano
    ADVANCED SYNTHESIS & CATALYSIS, 2006, 348 (15) : 2183 - 2190
  • [23] Physiological Role of D-amino Acids and Bioanalytical Potential of D-amino Acid Oxidases
    Tishkov V.I.
    Shelomov M.D.
    Pometun A.A.
    Savin S.S.
    Atroshenko D.L.
    Moscow University Chemistry Bulletin, 2023, 78 (1) : 1 - 9
  • [24] Activity of yeast D-amino acid oxidase on aromatic unnatural amino acids
    Caligiuri, Antonio
    D'Arrigo, Paola
    Rosini, Elena
    Pedrocchi-Fantoni, Giuseppe
    Tessaro, Davide
    Molla, Gianluca
    Servi, Stefano
    Pollegioni, Loredano
    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2008, 50 (2-4) : 93 - 98
  • [25] Mutant d-amino acid oxidase with higher catalytic efficiency toward d-amino acids with bulky side chains
    N. V. Komarova
    I. V. Golubev
    S. V. Khoronenkova
    V. I. Tishkov
    Russian Chemical Bulletin, 2012, 61 : 1489 - 1496
  • [26] Distribution of D-amino acids in vinegars and involvement of lactic acid bacteria in the production of D-amino acids
    Mutaguchi, Yuta
    Ohmori, Taketo
    Akano, Hirofumi
    Doi, Katsumi
    Ohshima, Toshihisa
    SPRINGERPLUS, 2013, 2
  • [27] Assays of D-Amino Acid Oxidase Activity
    Rosini, Elena
    Caldinelli, Laura
    Piubelli, Luciano
    FRONTIERS IN MOLECULAR BIOSCIENCES, 2018, 4
  • [28] ON REACTION MECHANISM OF D-AMINO ACID OXIDASE
    MASSEY, V
    FEDERATION PROCEEDINGS, 1964, 23 (2P1) : 486 - &
  • [29] FLAVIN CONTENT OF D-AMINO ACID OXIDASE
    YAGI, K
    OZAWA, T
    BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 81 (03) : 599 - &
  • [30] THE INHIBITION OF D-AMINO ACID OXIDASE BY DESOXYCORTICOSTERONE
    HAYANO, M
    DORFMAN, RI
    YAMADA, EY
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1950, 186 (02) : 603 - 614