Immobilization of glucose oxidase enzyme (GOD) in large pore ordered mesoporous cage-like FDU-1 silica

被引：10
|
作者：
Cides da Silva, L. C. [1 ]
Infante, C. M. C. [1 ]
Lima, A. W. O. [1 ]
Cosentino, I. C. [2 ]
Fantini, M. C. A. [3 ]
Rocha, F. R. P. [1 ]
Masini, J. C. [1 ]
Matos, J. R. [1 ]
机构：
[1] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, BR-05599970 Sao Paulo, Brazil
[2] IPEN, Ctr Ciencia & Tecnol Mat, BR-05422970 Sao Paulo, Brazil
[3] Univ Sao Paulo, Dept Fis Aplicada, Inst Fis, BR-05315970 Sao Paulo, Brazil
来源：
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2011年 / 70卷 / 3-4期
基金：
巴西圣保罗研究基金会;
关键词：
FDU-1; silica; Immobilized enzyme activity; Glucose oxidase; Flow injection; Chemiluminescence; MOLECULAR-SIEVES; ADSORPTION; STABILITY; COPOLYMER; TRIBLOCK; OXIDATION; SIZE; CD2+;
D O I：
10.1016/j.molcatb.2011.02.014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Large pore ordered mesoporous silica FDU-1 with three-dimensional (3D) face-centered cubic, Fm3m arrangement of rnesopores, was synthesized under strong acid media using B-50-6600 poly(ethylene oxide)-poly(butylene oxide)-poly(ethylene oxide) triblock copolymer (EO39BO47EO39), tetraethyl orthosilicate (TEOS) and trimethyl-benzene (TMB). Large pore FDU-1 silica was obtained by using the following gel composition 1TEOS:0.00735B50-6600:0.00735TMB:6HCl:155H(2)O. The pristine material exhibited a BET specific surface area of 684 m(2) g(-1), total pore volume of 0.89 cm(3) g(-1), external surface area of 49 m(2) g(-1) and microporous volume of 0.09 cm(3) g(-1). The enzyme activity was determined by the Flow Injection Analysis-Chemiluminescence (FIA-CL) method. For GOD immobilized on the FDU-1 silica, GOD supernatant and GOD solution, the FIA-CL results were 9.0, 18.6 and 34.0 U, respectively. The value obtained for the activity of the GOD solution with FIA-CL method is in agreement with the 35 U, obtained by spectrophotometry. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：149 / 153
页数：5
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