The crystals of a mannose-specific jacalin-related lectin from Morus nigra are merohedrally twinned

被引:4
|
作者
Rabijns, A
Verboven, C
de Armas, HN
Van Damme, EJM
Peumans, WJ
De Ranter, CJ
机构
[1] KU Leuven, Fac Farmaceut Wetenschappen, Lab Analyt Chem & Med Fysicochem, B-3000 Louvain, Belgium
[2] Katholieke Univ Leuven, Fac Landbouwkundige & Toegepaste Biol Wetenschapp, Lab Fytopathol & Plantenbescherming, B-3001 Louvain, Belgium
关键词
D O I
10.1107/S0907444901002104
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
MornigaM, a lectin from Morus nigra, belongs to the mannose-binding subgroup of the family of jacalin-related plant lectins. It was crystallized in the P6(5) space group, with unit-cell parameters a = b = 110.74, c = 159.28 Angstrom. The partially merohedrally twinned crystals could be detwinned and a subsequent molecular-replacement solution could be found using the coordinates of jacalin. Preliminary analysis clearly shows the tetrameric assembly of this protein. Furthermore, data from MornigaM crystals soaked in a mannose solution were collected.
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页码:609 / 611
页数:3
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