Structural analysis of the jacalin-related lectin MornigaM from the black mulberry (Morus nigra) in complex with mannose

被引：29
|
作者：
Rabijns, A
Barre, A
Van Damme, EJM
Peumans, WJ
De Ranter, CJ
Rougé, P
机构：
[1] CNRS, UMR 5546, F-31326 Castanet Tolosan, France
[2] Katholieke Univ Leuven, Fac Pharmaceut Sci, Lab Analyt Chem & Med Physicochem, Louvain, Belgium
[3] State Univ Ghent, Dept Mol Biotechnol, B-9000 Ghent, Belgium
来源：
FEBS JOURNAL | 2005年 / 272卷 / 14期
关键词：
carbohydrate-binding site; jacalin-related lectin; mannose; Morus nigra; quaternary association;
D O I：
10.1111/j.1742-4658.2005.04801.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structures of MornigaM and the MornigaM-mannose complex have been determined at 1.8 angstrom and 2.0 angstrom resolution, respectively. Both structures adopt the typical beta-prism motif found in other jacalin-related lectins and their tetrameric assembly closely resembles that of jacalin. The carbohydrate-binding cavity of MornigaM readily binds mannose. No major structural rearrangements can be observed in MornigaM upon binding of mannose. These results allow corroboration of the structure-function relationships within the small group of Moraceae lectins.
引用
收藏
页码：3725 / 3732
页数：8
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