Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells

被引：3

作者：

Talaikova, N. A. ^{[1
,2
]}

Popov, A. P. ^{[1
,3
,4
]}

Kalyanov, A. L. ^{[5
]}

Ryabukho, V. P. ^{[2
,6
]}

Meglinski, I. V. ^{[1
,3
,4
,7
]}

机构：

[1] Univ Oulu, Optoelect & Measurement Tech Res Unit, POB 4500, FI-90014 Oulu, Finland

[2] Saratov NG Chernyshevskii State Univ, Phys Fac, Astrachanskaya Str 83, Saratov 410012, Russia

[3] Natl Res Tomsk State Univ, Interdisciplinary Lab Biophoton, Tomsk 634050, Russia

[4] ITMO Univ, Kronverkskiy Prospect 49, St Petersburg 197101, Russia

[5] Univ Zurich Hosp, Clin Neonatol, CH-8091 Zurich, Switzerland

[6] Russian Acad Sci, Inst Precis Mech & Control, Rabochaya Str 24, Saratov 410028, Russia

[7] Irkutsk State Univ, Inst Biol, Irkutsk 66400, Russia

来源：

LASER PHYSICS LETTERS | 2017年 / 14卷 / 10期

基金：

俄罗斯科学基金会;

关键词：

diffraction phase microscopy; refractive index; micro-interferometry; red blood cells; cell imaging; quantitative phase imaging; REFRACTIVE-INDEX; DYNAMICS; BLOOD; INTERFEROMETRY; LIGHT;

D O I：

10.1088/1612-202X/aa7d38

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.

引用

页数：7

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