Proteolytic activity in Fasciola hepatica is reduced by the administration of cathepsin L mimotopes

被引：11
|
作者：
Villa-Mancera, A. [1 ,2 ]
Quiroz-Romero, H. [2 ]
Correa, D. [4 ]
Alonso, R. A. [3 ]
机构：
[1] Benemerita Univ Autonoma Puebla, Fac Vet Med & Zootecnia, Lab Genet & Reprod, Tecamachalco Puebla 75482, Mexico
[2] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Parasitol, Mexico City 04510, DF, Mexico
[3] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Genet & Bioestadist, Mexico City 04510, DF, Mexico
[4] Inst Nacl Pediatria, Lab Inmunol Expt, Mexico City 04530, DF, Mexico
来源：
JOURNAL OF HELMINTHOLOGY | 2011年 / 85卷 / 01期
关键词：
PHAGE DISPLAY; PROTEINASES; VACCINATION; INDUCTION; INFECTION; PROTEASES; VACCINES; IMMUNITY; CATTLE; SHEEP;
D O I：
10.1017/S0022149X1000026X
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The objective of this study was to assess the proteolytic activity of Fasciola hepatica cathepsins in liver sections from mice vaccinated with phage clones of cathepsin L mimotopes, using the film in situ zymography technique. Female BALB/c mice were immunized three times with 2.5 x 10(11) phage particles without adjuvant. Animals vaccinated with phage clones produced high titres of anti-mimotope antibodies and a significant reduction in fluke burden was observed following challenge with metacercariae of F. hepatica. The proteolytic activity in hepatic tissue was reduced after the immunization with phage clones.
引用
收藏
页码：51 / 55
页数：5
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