The effect of 5′-(N,N-dimethyl)-amiloride on cytotoxic activity of doxorubicin and vincristine in CEM cell lines

被引：0

作者：

Radváková, I ^{[1
]}

Mirossay, A ^{[1
]}

Mojzis, J ^{[1
]}

Mirossay, L ^{[1
]}

机构：

[1] Safarik Univ, Fac Med, Dept Pharmacol, Tr SNP 1, Kosice 04066, Slovakia

来源：

PHYSIOLOGICAL RESEARCH | 2001年 / 50卷 / 03期

关键词：

amiloride; CEM cell lines; anticancer drugs; resistance; intracellular pH;

D O I：

暂无

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Intracellular pH (pH(i)) plays an important role in anticancer drug accumulation in cancer cells. Resistant cells often express membrane P-glycoprotein responsible for active drug extrusion and participating in increased pHi. In the present paper, we report on the influence of Na+/H+-exchanger inhibitor, 5'-(N,N-dimethyl)-amiloride (AMI), on the cytotoxic effects of doxorubicin (DOXO) and vincristine (VCR) in the parental CEM, and resistant CEM/DNR and CEM/VCR cell lines. The obtained results revealed a potentiating effect of AMI to both anticancer drugs in parental CEM line. However, AMI did not significantly potentiate the effect of DOXO or VCR in resistant CEM cell lines. We conclude, that inhibition of Na+/H+-exchanger by AMI is not sufficient for reversal of drug resistance in the tested CEM/DNR and CEM/VCR cell lines and the possible change in pH(i) does not affect the mechanisms of cell resistance.

引用

页码：283 / 287

页数：5

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