Evaluation of free radical scavenging and antioxidant activities of polydatin nanoethosomes

Cellular damage induced by free-radicals like reactive oxygen species has been implicated in several diseases. The ROS radicals like alkoxy radical(RO-) and peroxy radical (ROO-) are similar to those that are physiologically active and thus might initiate a cascade of intracellular toxic events leading to DNA damage and cell death. Hence naturally anti-oxidant play a vital role in combating these conditions. In this study, polydatin nanoethosomes (Pol-NE) was prepared by high pressure homogenization technique. The effects of Pol-NE on free radical scavenging and antioxidant was investigated. The particle size and zeta potential of Pol-NE was 96.1 +/- 4.5 nm and -17.31 +/- 1.67 mV, respectively. By free radical scavenging and antioxidant assays, the IC50 value of Pol-NE were 28.71, 9.83 mu g/mL with DPPH, ABTS assay respectively, and 0.143 mg ferrous sulfate/1 mg Pol-NE with FRAP assay. These results indicated that the antioxidant properties of Pol-NE hold great potential used as an alternative to more toxic synthetic anti-oxidants as an additive in food, cosmetic and pharmaceutical preparations for the oxidative diseases treatment.