Salivary cortisol as a marker of acute stress in dogs: a review

Public interest in the welfare of domestic dogs has increased in recent years. Dogs under human care should experience as little stress as possible, and as such it is necessary to measure and quantify their levels of stress. Stress parameters that can be measured noninvasively may help to identify the poor welfare of animals. This review aimed to determine whether and under what conditions the hormone cortisol in dog saliva can be used as a noninvasive acute stress marker. The use of salivary cortisol as a stress marker has some disadvantages, which can lead to data misinterpretations. A key factor is the standardized method of sampling and subsequent processing before analysis. In addition, possible circadian alternation and individual variability of cortisol hormone levels should be consistently considered during the preparation of the experimental scheme, statistical data processing and final interpretation of the results. Because of the complex nature of the stress response, the observation of salivary cortisol should be supplemented with behavioral observations, but it should be noted that behavioral stress symptoms may not always be positively correlated with stress hormone production. Besides behavioral observations, it is advisable to supplement the measurement of cortisol by other salivary stress markers of sympathetic-adrenal-medullary and hypothalamic-pituitary-adrenal pathways. This comprehensive assessment of the stress impact on the individual will enable one to characterize the level and type of stress. (C) 2019 Elsevier Inc. All rights reserved.