Effect of hyperthermia on invasion ability and TGF-β1 expression of breast carcinoma MCF-7 cells

被引：33
|
作者：
Xie, Xiaoxue [1 ,2 ]
Shao, Xunfan [3 ]
Gao, Fuping [1 ,2 ]
Jin, Hekun [1 ,2 ,4 ]
Zhou, Jumei [1 ,2 ,4 ]
Du, Lehui [1 ,2 ]
Zhang, Yingying [1 ,2 ]
Ouyang, Weiwei [1 ,2 ]
Wang, Xiaowen [1 ,2 ]
Zhao, Lingyun [1 ,2 ]
Zhang, Xiaodong [1 ,2 ]
Tang, Jintian [1 ,2 ]
机构：
[1] Tsinghua Univ, Inst Med Phys & Engn, Dept Engn Phys, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Key Lab Particle & Radiat Imaging, Minist Educ, Beijing 100084, Peoples R China
[3] Guangzhou Med Univ, Affiliated Tumor Hosp, Guangzhou 510095, Guangdong, Peoples R China
[4] Hunan Prov Tumor Hosp, Dept Radiotherapy, Changsha, Hunan, Peoples R China
来源：
ONCOLOGY REPORTS | 2011年 / 25卷 / 06期
基金：
中国国家自然科学基金;
关键词：
hyperthermia; MCF-7; cells; cancer; invasion; 1-MATRIX METALLOPROTEINASE PRODUCTION; ENDOTHELIAL GROWTH-FACTOR; TGF-BETA; TRANSFORMING GROWTH-FACTOR-BETA-1; TUMOR PROGRESSION; METASTASIS; PROGELATINASE; FIBROBLASTS; SUPPRESSION; PROGNOSIS;
D O I：
10.3892/or.2011.1240
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The present study aimed to investigate heating-induced alterations of breast cancer cell invasion abilities and the potential mechanisms associated with TGF-beta(1) expression. MCF-7 cells were heated at 43, 45, 47 and 37 degrees C for 30 min. In vitro cell invasion ability was evaluated by matrigel invasion assay. The activity of matrix metalloproteinase (MMP)-2/9 was investigated by gelatin zymographic assays. Expression of vascular endothelial growth factor (VEGF) and transforming growth factor-beta(1) (TGF-beta(1)) was investigated by immunocytochemistry and RT-PCR. Apoptosis was analysed by flow-cytometry. The invasive potential of MCF-7 cells was reduced by heating, and MMP-2/9 secretion and enzymatic activity were suppressed. Furthermore, VEGF and TGF-beta(1) mRNA and proteins were suppressed by hyperthermia. These results suggest that down-regulation of the expression of TGF-beta(1), EGF and MMPs by hyperthermia probably accounts for the inhibition of the invasive abilities of MCF-7 cells.
引用
下载
收藏
页码：1573 / 1579
页数：7
相关论文
共 50 条
  • [21] 17β-estradiol enhances chemotactic motility of human breast cancer MCF-7 cells, but inhibits TGF-β induced migration
    Malek, D
    Kleuser, B
    Gust, R
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2005, 371 : R65 - R65
  • [22] The effect of CBG (BDS) botanical cannabinoid extract on MCF-7 human breast carcinoma cells
    Javid, Farideh
    Fshinjavid, Saeed
    FASEB JOURNAL, 2013, 27
  • [23] Expression of adenosine receptors in MCF-7 human breast cancer cells
    Clark, JH
    Broadley, KJ
    Hutcheson, IR
    Nicholson, RI
    Kidd, EJ
    BRITISH JOURNAL OF PHARMACOLOGY, 2003, 138 : U65 - U65
  • [24] Differential effect of glucose deprivation on MAPK activation in drug sensitive human breast carcinoma MCF-7 and multidrug resistant MCF-7/ADR cells
    Anjali K. Gupta
    Yong J. Lee
    Sandra S. Galoforo
    Christine M. Berns
    Alvaro A. Martinez
    Peter M. Corry
    Xiao-yu Wu
    Kun-Liang Guan
    Molecular and Cellular Biochemistry, 1997, 170 : 23 - 30
  • [25] Investigation of Gallic Acid Induced Anticancer Effect in Human Breast Carcinoma MCF-7 Cells
    Wang, Ke
    Zhu, Xue
    Zhang, Kai
    Zhu, Ling
    Zhou, Fanfan
    JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2014, 28 (09) : 387 - 393
  • [26] Artemether effect and its interaction with vincristine and doxorubicin on human breast carcinoma MCF-7 cells
    Samandari-Bahraseman, Mohammad Rasoul
    Sarhadi, Solmaz
    Esmaeili-Mahani, Saeed
    PHYSIOLOGY AND PHARMACOLOGY, 2016, 20 (02): : 117 - 121
  • [27] Differential effect of glucose derivation on MAPK activation in drug sensitive human breast carcinoma MCF-7 and multidrug resistant MCF-7/ADR cells
    Gupta, AK
    Lee, YJ
    Galoforo, SS
    Berns, CM
    Martinez, AA
    Corry, PM
    Wu, XY
    Guan, KL
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 1997, 170 (1-2) : 23 - 30
  • [28] The effect of anastrozole on mRNA expression of oestrogen related gene in MCF-7 breast cancer cells
    宋张骏
    吴怡
    马清涌
    Military Medical Research, 2006, (03) : 178 - 181
  • [29] Effect of SMYD3 on the microRNA expression profile of MCF-7 breast cancer cells
    Chen, Dongju
    Liu, Lei
    Luo, Xuegang
    Mu, Ai
    Yan, Lihua
    Chen, Xiaoying
    Wang, Lei
    Wang, Nan
    He, Hongpeng
    Zhou, Hao
    Zhang, Tongcun
    ONCOLOGY LETTERS, 2017, 14 (02) : 1831 - 1840
  • [30] The effect of the new SERM arzoxifene on growth and gene expression in MCF-7 breast cancer cells
    Freddie, CT
    Larsen, SS
    Bartholomæussen, M
    Lykkesfeldt, AE
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2004, 219 (1-2) : 27 - 36
12345