Effect of hyperthermia on invasion ability and TGF-β1 expression of breast carcinoma MCF-7 cells

被引：32
|
作者：
Xie, Xiaoxue [1 ,2 ]
Shao, Xunfan [3 ]
Gao, Fuping [1 ,2 ]
Jin, Hekun [1 ,2 ,4 ]
Zhou, Jumei [1 ,2 ,4 ]
Du, Lehui [1 ,2 ]
Zhang, Yingying [1 ,2 ]
Ouyang, Weiwei [1 ,2 ]
Wang, Xiaowen [1 ,2 ]
Zhao, Lingyun [1 ,2 ]
Zhang, Xiaodong [1 ,2 ]
Tang, Jintian [1 ,2 ]
机构：
[1] Tsinghua Univ, Inst Med Phys & Engn, Dept Engn Phys, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Key Lab Particle & Radiat Imaging, Minist Educ, Beijing 100084, Peoples R China
[3] Guangzhou Med Univ, Affiliated Tumor Hosp, Guangzhou 510095, Guangdong, Peoples R China
[4] Hunan Prov Tumor Hosp, Dept Radiotherapy, Changsha, Hunan, Peoples R China
来源：
ONCOLOGY REPORTS | 2011年 / 25卷 / 06期
基金：
中国国家自然科学基金;
关键词：
hyperthermia; MCF-7; cells; cancer; invasion; 1-MATRIX METALLOPROTEINASE PRODUCTION; ENDOTHELIAL GROWTH-FACTOR; TGF-BETA; TRANSFORMING GROWTH-FACTOR-BETA-1; TUMOR PROGRESSION; METASTASIS; PROGELATINASE; FIBROBLASTS; SUPPRESSION; PROGNOSIS;
D O I：
10.3892/or.2011.1240
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The present study aimed to investigate heating-induced alterations of breast cancer cell invasion abilities and the potential mechanisms associated with TGF-beta(1) expression. MCF-7 cells were heated at 43, 45, 47 and 37 degrees C for 30 min. In vitro cell invasion ability was evaluated by matrigel invasion assay. The activity of matrix metalloproteinase (MMP)-2/9 was investigated by gelatin zymographic assays. Expression of vascular endothelial growth factor (VEGF) and transforming growth factor-beta(1) (TGF-beta(1)) was investigated by immunocytochemistry and RT-PCR. Apoptosis was analysed by flow-cytometry. The invasive potential of MCF-7 cells was reduced by heating, and MMP-2/9 secretion and enzymatic activity were suppressed. Furthermore, VEGF and TGF-beta(1) mRNA and proteins were suppressed by hyperthermia. These results suggest that down-regulation of the expression of TGF-beta(1), EGF and MMPs by hyperthermia probably accounts for the inhibition of the invasive abilities of MCF-7 cells.
引用
收藏
页码：1573 / 1579
页数：7
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