CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis

被引:5
|
作者
Yang, Jian [1 ]
Tian, Shuaiwei [1 ]
Wang, Baocheng [1 ]
Wang, Jiajia [1 ]
Cao, Liangliang [1 ]
Wang, Qinhua [1 ]
Xie, Wanqun [1 ]
Liang, Zhuangzhuang [1 ]
Zhao, Heng [1 ]
Zhao, Yang [1 ]
Liao, Keman [2 ,3 ]
Ma, Jie [1 ]
机构
[1] Shanghai Jiao Tong Univ, Xinhua Hosp, Sch Med, Dept Pediat Neurosurg, Shanghai, Peoples R China
[2] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Brain Injury Ctr, Shanghai, Peoples R China
[3] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Neurosurg, Shanghai, Peoples R China
来源
FRONTIERS IN ONCOLOGY | 2021年 / 11卷
基金
美国国家科学基金会;
关键词
glioblastoma; miR-877-5p; FOXM1; competing endogenous RNA (CeRNA); CELL LUNG-CANCER; MESENCHYMAL TRANSITION; PROMOTES TUMORIGENESIS; FOXM1; EXPRESSION; METASTASIS; INVASION; GROWTH; LOOP;
D O I
10.3389/fonc.2021.801776
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Glioblastoma is a rare yet lethal type of tumor that poses a crucible for the medical profession, owing to its rapid proliferation and invasion resulting in poor prognosis. Circular RNAs (circRNAs), a subclass of regulatory RNAs, are implicated in the regulation of cancerous progression. This study aims to investigate the roles and underlying mechanism of circPIK3C2A in regulating proliferation and invasion of glioblastoma. qRT-PCR assays showed that the expression level of circPIK3C2A was aberrantly higher in glioblastoma cell lines, in comparison with that in normal glia cells. The ectopic expression of circPIK3C2A promoted the proliferation, invasion and clonal formation of glioblastoma cells, while circPIK3C2A loss-of-function exerted exactly the opposite biological effects on the cells. The construction of subcutaneous xenograft tumor model in nude mice indicated that circPIK3C2A loss-of-function effectively diminished tumor load in vivo and prolonged the survival time of tumor-bearing animals. Luciferase reporter assay confirmed the interaction among circPIK3C2A/miR-877-5p and FOXM1. CircPIK3C2A function as competitive endogenous RNA via sponging miR-877-5p through certain binding sites, thereby modulating the expression of FOXM1. Our results collectively indicate that circPIK3C2A functions as ceRNA by mediating miR-877-5p/FOXM1 axis, providing a novel perspective of applying CircPIK3C2A in the clinical intervention of glioblastoma in the future.
引用
收藏
页数:13
相关论文
共 50 条
  • [21] Inhibition of LINC00958 hinders the progression of osteoarthritis through regulation of the miR-214-3p/FOXM1 axis
    Yingchuan Yin
    Qiaojuan He
    Jing He
    Ying Feng
    Yunyun Xu
    [J]. Journal of Orthopaedic Surgery and Research, 19
  • [22] Long non-coding RNA DSCAM-AS1 contributes to the tumorigenesis of cervical cancer by targeting miR-877-5p/ATXN7L3 axis
    Liang, Jie
    Zhang, Shujuan
    Wang, Wei
    Xu, Yan
    Kawuli, Atikan
    Lu, Jiaqi
    Xiu, Xuemei
    [J]. BIOSCIENCE REPORTS, 2020, 40
  • [23] Inhibition of LINC00958 hinders the progression of osteoarthritis through regulation of the miR-214-3p/FOXM1 axis
    Yin, Yingchuan
    He, Qiaojuan
    He, Jing
    Feng, Ying
    Xu, Yunyun
    [J]. JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH, 2024, 19 (01)
  • [24] The novel circCLK3/miR-320a/FoxM1 axis promotes cervical cancer progression
    Hanqing Hong
    Hai Zhu
    Shujun Zhao
    Kaili Wang
    Nan Zhang
    Yun Tian
    Yan Li
    Yaping Wang
    Xiaofeng Lv
    Tianxiang Wei
    Yan Liu
    Suzhen Fan
    Yang Liu
    Yuan Li
    Aojie Cai
    Shuo Jin
    Qiaohong Qin
    Hongyu Li
    [J]. Cell Death & Disease, 10
  • [25] The novel circCLK3/miR-320a/FoxM1 axis promotes cervical cancer progression
    Hong, Hanqing
    Zhu, Hai
    Zhao, Shujun
    Wang, Kaili
    Zhang, Nan
    Tian, Yun
    Li, Yan
    Wang, Yaping
    Lv, Xiaofeng
    Wei, Tianxiang
    Liu, Yan
    Fan, Suzhen
    Liu, Yang
    Li, Yuan
    Cai, Aojie
    Jin, Shuo
    Qin, Qiaohong
    Li, Hongyu
    [J]. CELL DEATH & DISEASE, 2019, 10 (12)
  • [26] CircRIP2 promotes NSCLC progression by sponging for miR-671-5p to regulate FOXM1 expression
    Liu, Yizhi
    Feng, Xing
    Kang, Shuhong
    Lv, Feng
    Ni, Yunfeng
    Wu, Hua
    [J]. HISTOLOGY AND HISTOPATHOLOGY, 2022, 37 (02) : 117 - 124
  • [27] LncRNA SNHG16 Promotes the Progression of Laryngeal Squamous Cell Carcinoma by Mediating miR-877-5p/FOXP4 Axis
    Wang, Xiaoli
    Liu, Liming
    Zhao, Wenfei
    Li, Qingyan
    Wang, Guangsheng
    Li, Huahui
    [J]. ONCOTARGETS AND THERAPY, 2020, 13 : 4569 - 4579
  • [28] CircPIP5K1A facilitates gastric cancer progression via miR-376c-3p/ZNF146 axis
    Ma, Yan
    Cong, Xiliang
    Zhang, Yiyun
    Yin, Xin
    Zhu, Ziyu
    Xue, Yingwei
    [J]. CANCER CELL INTERNATIONAL, 2020, 20 (01)
  • [29] CircPIP5K1A facilitates gastric cancer progression via miR-376c-3p/ZNF146 axis
    Yan Ma
    Xiliang Cong
    Yiyun Zhang
    Xin Yin
    Ziyu Zhu
    Yingwei Xue
    [J]. Cancer Cell International, 20
  • [30] Circular PVT1 regulates cell proliferation and invasion via miR-149-5p/FOXM1 axis in ovarian cancer
    Li, Min
    Chi, Chi
    Zhou, Liqin
    Chen, Youguo
    Tang, Xiuwu
    [J]. JOURNAL OF CANCER, 2021, 12 (02): : 611 - 621