MOSFET-based pulse power supply for bacterial transformation

被引：14

作者：

Grenier, Jason R. ^{[1
]}

Jayaram, Shesha H. ^{[1
]}

Kazerani, Mehrdad ^{[1
]}

Wang, Haifeng ^{[2
]}

Griffiths, Mansel W. ^{[2
]}

机构：

[1] Univ Waterloo, Dept Elect & Comp Engn, Waterloo, ON N2L 3G1, Canada

[2] Univ Guelph, Canadian Res Inst Food Safety, Guelph, ON N1G 2W1, Canada

来源：

IEEE TRANSACTIONS ON INDUSTRY APPLICATIONS | 2008年 / 44卷 / 01期

关键词：

bacterial transformation; DNA transfusion; electroporation; Escherichia coli;

D O I：

10.1109/TIA.2007.912762

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this paper, a MOSFET-based pulsed power supply capable of supplying square pulses of up to 3000 V and widths from nanoseconds to milliseconds is presented and used for an investigation into electroporation-mediated delivery of a plasmid DNA molecule into the pathogenic bacterium Escherichia coli O157:H7. It was concluded that increasing the electric field strength and pulse amplitude resulted in an increase in the number of transformants. However, increasing the number of pulses had the effect of reducing the number of transformants. In all the experiments, the number of cells that were inactivated by the exposure to the pulsed electric field were also measured.

引用

页码：25 / 31

页数：7

共 50 条

[21] A MOSFET-based model of a class 2 nerve membrane
Kohno, T
Aihara, K
[J]. IEEE TRANSACTIONS ON NEURAL NETWORKS, 2005, 16 (03): : 754 - 773
[22] Characterization of buildup effects in OneDosePlus MOSFET-based dosimeters
Pursley, J.
Beyer, G. P.
Craciunescu, O.
Fraisse, C.
Carroll, J.
Oldham, M.
[J]. MEDICAL PHYSICS, 2007, 34 (06) : 2423 - 2423
[23] MOSFET-based high voltage double square-wave pulse generator with an inductive adder configuration
Zhao, Xin
Zhang, Qiaogen
Long, Jinghua
Lei, Yunfei
Liu, Jinyuan
[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2015, 793 : 12 - 17
[24] Active Power Filter Capability of a 10 kV SiC MOSFET-Based Asynchronous Microgrid Power Conditioning System
Li, Dingrui
Nie, Cheng
Huang, Xingxuan
Lin, Min
Wang, Fred
Tolbert, Leon M.
[J]. 2023 IEEE APPLIED POWER ELECTRONICS CONFERENCE AND EXPOSITION, APEC, 2023, : 137 - 143
[25] Mitigation of Process Variation in MOSFET-Based Narrowband LNA
Suresh, D.
Kumar, N. Vinodh
Nagarajan, K. K.
Srinivasan, R.
[J]. 2014 INTERNATIONAL CONFERENCE ON INFORMATION COMMUNICATION AND EMBEDDED SYSTEMS (ICICES), 2014,
[26] MOSFET-based, analog circuit calculates square root
Lahiri, Abhirup
[J]. EDN, 2009, 54 (04) : 52 - +
[27] A SiC MOSFET-based parallel multi-inverter inductive power transfer (IPT) system
Bo, Qiang
Wang, Lifang
Zhang, Yuwang
[J]. JOURNAL OF POWER ELECTRONICS, 2022, 22 (06) : 1047 - 1057
[28] Thin-Film MOSFET-Based Pressure Sensor
Gardner, Ethan L. W.
De Luca, Andrea
Philippe, Julien
Dragomirescu, Daniela
Udrea, Florin
[J]. IEEE SENSORS LETTERS, 2019, 3 (07)
[29] AN IMPROVED MOSFET-BASED ROBINSON OSCILLATOR FOR NMR DETECTION
WILSON, KJ
VALLABHAN, CPG
[J]. MEASUREMENT SCIENCE AND TECHNOLOGY, 1990, 1 (05) : 458 - 460
[30] Stability of MOSFET-Based Electronic Components in Wearable and Implantable Systems
Jin, Xin
Jiang, Chunsheng
Song, Enming
Fang, Hui
Rogers, John A.
Alam, Muhammad Ashraful
[J]. IEEE TRANSACTIONS ON ELECTRON DEVICES, 2017, 64 (08) : 3443 - 3451

← 1 2 3 4 5 →