VALIDATION AND UNCERTAINTY ESTIMATION OF A METHOD BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR THE ANALYSIS OF LYCOPENE AND β-CAROTENE IN TOMATO PULP

VALIDATION AND UNCERTAINTY ESTIMATION OF A METHOD BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR THE ANALYSIS OF LYCOPENE AND beta-CAROTENE IN TOMATO PULP. A method to quantify lycopene and beta-carotene in freeze dried tomato pulp by high performance liquid chromatography (HLPC) was validated according to the criteria of selectivity, sensitivity, precision and accuracy. and uncertainty estimation of measurement was determined with data obtained in the validation. The validated method presented is selective in terms of analysis, and it had a good precision and accuracy. Detection limit for lycopene and beta-carotene was 4.2 and 0.23 mg 100 g(-1), respectively. The estimation of expanded uncertainty (K = 2) for lycopene was 104 +/- 21 mg 100 g(-1) and for beta-carotene was 6.4 +/- 1.5 mg 100 g(-1).