Genetic Transformation of a Seedless Grape Cultivar 'Autumn Royal' (Vitis vinifera L.)

被引：0

作者：

Jittayasothorn, Y. ^{[1
]}

Lu, J. ^{[1
]}

Xu, X. ^{[1
]}

Thipyapong, P. ^{[2
]}

Boonkerd, N. ^{[3
]}

机构：

[1] Florida A&M Univ, CESTA, Ctr Viticulture & Small Fruit Res, Tallahassee, FL 32317 USA

[2] Sch Crop Prod Technol, Bangkok, Thailand

[3] Suranaree Univ Technol, Inst Agr Technol, Sch Biotechnol, 30000 Nakhon Ratchasima, Thailand

来源：

IX INTERNATIONAL CONFERENCE ON GRAPE GENETICS AND BREEDING | 2009年 / 827卷

关键词：

transformation; GFP; grape; Agrobacterium tumefaciens; Vitis vinifera; EXPRESSION; CULTURE; GROWTH;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Agrobacterium-mediated transformation was developed for 'Autumn Royal Seedless' with SPEMs or somatic embryo as the target materials. No transformed cells were obtained when we used SPEMs as target cells. GFP was very helpful to monitor transformed cells. Two months after transformation, some embryogenic calli and globular somatic embryos showed fluorescent signal on selection medium. The somatic embryos did not grow beyond the globular stage. Using this protocol with 'Autumn Royal Seedless', putative transgenic somatic embryos were produced in low transformation frequency.

引用

页码：405 / 408

页数：4

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