Transmembrane Ca2+ gradient is essential for high anion transport activity of human erythrocytes

被引:2
|
作者
Tu, YP
Feng, C
Xu, H
Guang, ZY
Lu, QW
Yang, FY
机构
[1] ACAD SINICA,NATL LAB BIOMACROMOL,BEIJING 100101,PEOPLES R CHINA
[2] ACAD SINICA,INST BIOPHYS,CTR EXPT TECHNOL,BEIJING 100101,PEOPLES R CHINA
关键词
transmembrane Ca2+ gradient; anion transport activity; Band; 3; freeze-fracture; resealed erythrocyte ghosts;
D O I
10.1007/BF01855014
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The role of a transmembrane Ca2+ gradient in anion transport by Band 3 of human resealed erythrocyte ghosts has been studied. The results show that a transmembrane Ca2+ gradient is essential for the conformation of erythrocyte Band 3 with higher anion transport activity. The dissipation of the transmembrane Ca2+ gradient by the ionophore A23187 inhibits the anion transport activity. The extent of this inhibition approaches 90% as the Ca2+ concentration on both sides of the ghost membrane is increased to 1.0 mM and half-maximum inhibition is observed at 0.25 mM Ca2+. Addition of ATP (0.4 mM) to the resealing medium can partly reestablish the transmembrane Ca2+ gradient by activation of Ca2+-ATPase and alleviate the inhibition to some extent. N-ethylmaleimide, an inhibitor of erythrocyte Ca2+-ATPase, prevents such restoration. Electron micrographs reveal that numerous larger intramembranous particles can be observed on the P-faces of freeze-fractured resealed ghosts in the absence of a transmembrane Ca2+ gradient.
引用
收藏
页码:299 / 311
页数:13
相关论文
共 50 条
  • [41] CA2+ ATPASE ACTIVITY IN HUMAN HYPERTENSION
    MCCARRON, D
    MORRIS, C
    VINCENZI, F
    HYPERTENSION, 1984, 6 (05) : 781 - 781
  • [42] CA2+ TRANSPORT IN UNSTIMULATED PLATELETS OF ESSENTIAL HYPERTENSIVES AND THEIR BLOOD RELATIVES
    GULATI, S
    KHULLAR, M
    SAGAR, S
    GANGULY, NK
    SHARMA, BK
    JOURNAL OF HYPERTENSION, 1992, 10 (10) : 1213 - 1219
  • [43] Ca2+ efflux facilitated by co-transport of inorganic phosphate anion in the H+/Ca2+ antiporter YfkE
    Niu, Wei
    Zhou, Wenchang
    Lu, Shuo
    Vu, Trung
    Jayaraman, Vasanthi
    Faraldo-Gomez, Jose D.
    Zheng, Lei
    COMMUNICATIONS BIOLOGY, 2023, 6 (01)
  • [44] Ca2+ efflux facilitated by co-transport of inorganic phosphate anion in the H+/Ca2+ antiporter YfkE
    Wei Niu
    Wenchang Zhou
    Shuo Lu
    Trung Vu
    Vasanthi Jayaraman
    José D. Faraldo-Gómez
    Lei Zheng
    Communications Biology, 6
  • [45] INHIBITION OF ANION TRANSPORT IN HUMAN-ERYTHROCYTES BY CARBODIIMIDES
    CRAIK, JD
    REITHMEIER, RAF
    BIOPHYSICAL JOURNAL, 1984, 45 (02) : A199 - A199
  • [46] CATABOLISM OF THE ANION TRANSPORT PROTEIN IN HUMAN-ERYTHROCYTES
    MORRISON, M
    GRANT, W
    SMITH, HT
    MUELLER, TJ
    HSU, L
    BIOCHEMISTRY, 1985, 24 (22) : 6311 - 6315
  • [47] EFFECTS OF A CA2+ GRADIENT AND WATER ACTIVITY ON THE PHOSPHORYLATION OF CA2+-ATPASE BY PI
    CALDEIRA, MT
    DEMEIS, L
    FEBS LETTERS, 1991, 288 (1-2) : 10 - 12
  • [48] Effect of transmembrane Ca2+ gradient on the coupling of beta-adrenergic receptors and adenylyl cyclase
    Fan, GF
    Yang, XY
    Huang, YG
    Yang, FY
    BIOSCIENCE REPORTS, 1996, 16 (04) : 327 - 341
  • [49] Variations in transmembrane Ca2+ gradient and apoptosis of macrophages induced by oxidized low density lipoprotein
    Yang, XY
    Zhang, YZ
    Huang, YG
    Yang, FY
    BIOSCIENCE REPORTS, 2001, 21 (05) : 667 - 681
  • [50] Effect of transmembrane Ca2+ gradient on ligand binding of reconstituted beta-adrenergic receptors
    Yang, XY
    Fan, GF
    Huang, YG
    Yang, FY
    CHINESE SCIENCE BULLETIN, 1996, 41 (14): : 1214 - 1218